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Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance

Intestinal inflammation changes the luminal habitat for microbes through mechanisms that have not been fully resolved. We noticed that the FepE regulator of very long O-antigen chain assembly in the enteric pathogen Salmonella enterica serotype Typhimurium (S. Typhimurium) conferred a luminal fitnes...

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Autores principales: Crawford, Robert W., Keestra, A. Marijke, Winter, Sebastian E., Xavier, Mariana N., Tsolis, Renée M., Tolstikov, Vladimir, Bäumler, Andreas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447750/
https://www.ncbi.nlm.nih.gov/pubmed/23028318
http://dx.doi.org/10.1371/journal.ppat.1002918
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author Crawford, Robert W.
Keestra, A. Marijke
Winter, Sebastian E.
Xavier, Mariana N.
Tsolis, Renée M.
Tolstikov, Vladimir
Bäumler, Andreas J.
author_facet Crawford, Robert W.
Keestra, A. Marijke
Winter, Sebastian E.
Xavier, Mariana N.
Tsolis, Renée M.
Tolstikov, Vladimir
Bäumler, Andreas J.
author_sort Crawford, Robert W.
collection PubMed
description Intestinal inflammation changes the luminal habitat for microbes through mechanisms that have not been fully resolved. We noticed that the FepE regulator of very long O-antigen chain assembly in the enteric pathogen Salmonella enterica serotype Typhimurium (S. Typhimurium) conferred a luminal fitness advantage in the mouse colitis model. However, a fepE mutant was not defective for survival in tissue, resistance to complement or resistance to polymyxin B. We performed metabolite profiling to identify changes in the luminal habitat that accompany S. Typhimurium-induced colitis. This analysis suggested that S. Typhimurium-induced colitis increased the luminal concentrations of total bile acids. A mutation in fepE significantly reduced the minimal inhibitory concentration (MIC) of S. Typhimurium for bile acids in vitro. Oral administration of the bile acid sequestrant cholestyramine resin lowered the concentrations of total bile acids in colon contents during S. Typhimurium infection and significantly reduced the luminal fitness advantage conferred by the fepE gene in the mouse colitis model. Collectively, these data suggested that very long O-antigen chains function in bile acid resistance of S. Typhimurium, a property conferring a fitness advantage during luminal growth in the inflamed intestine.
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spelling pubmed-34477502012-10-01 Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance Crawford, Robert W. Keestra, A. Marijke Winter, Sebastian E. Xavier, Mariana N. Tsolis, Renée M. Tolstikov, Vladimir Bäumler, Andreas J. PLoS Pathog Research Article Intestinal inflammation changes the luminal habitat for microbes through mechanisms that have not been fully resolved. We noticed that the FepE regulator of very long O-antigen chain assembly in the enteric pathogen Salmonella enterica serotype Typhimurium (S. Typhimurium) conferred a luminal fitness advantage in the mouse colitis model. However, a fepE mutant was not defective for survival in tissue, resistance to complement or resistance to polymyxin B. We performed metabolite profiling to identify changes in the luminal habitat that accompany S. Typhimurium-induced colitis. This analysis suggested that S. Typhimurium-induced colitis increased the luminal concentrations of total bile acids. A mutation in fepE significantly reduced the minimal inhibitory concentration (MIC) of S. Typhimurium for bile acids in vitro. Oral administration of the bile acid sequestrant cholestyramine resin lowered the concentrations of total bile acids in colon contents during S. Typhimurium infection and significantly reduced the luminal fitness advantage conferred by the fepE gene in the mouse colitis model. Collectively, these data suggested that very long O-antigen chains function in bile acid resistance of S. Typhimurium, a property conferring a fitness advantage during luminal growth in the inflamed intestine. Public Library of Science 2012-09-20 /pmc/articles/PMC3447750/ /pubmed/23028318 http://dx.doi.org/10.1371/journal.ppat.1002918 Text en © 2012 Crawford et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Crawford, Robert W.
Keestra, A. Marijke
Winter, Sebastian E.
Xavier, Mariana N.
Tsolis, Renée M.
Tolstikov, Vladimir
Bäumler, Andreas J.
Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title_full Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title_fullStr Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title_full_unstemmed Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title_short Very Long O-antigen Chains Enhance Fitness during Salmonella-induced Colitis by Increasing Bile Resistance
title_sort very long o-antigen chains enhance fitness during salmonella-induced colitis by increasing bile resistance
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447750/
https://www.ncbi.nlm.nih.gov/pubmed/23028318
http://dx.doi.org/10.1371/journal.ppat.1002918
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