Activation Biosensor for G Protein-Coupled Receptors: A FRET-Based m1 Muscarinic Activation Sensor That Regulates G(q)

We describe the design, construction and validation of a fluorescence sensor to measure activation by agonist of the m1 muscarinic cholinergic receptor, a prototypical class I G(q)-coupled receptor. The sensor uses an established general design in which Förster resonance energy transfer (FRET) from...

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Detalles Bibliográficos
Autores principales: Chang, Seungwoo, Ross, Elliott M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447775/
https://www.ncbi.nlm.nih.gov/pubmed/23029161
http://dx.doi.org/10.1371/journal.pone.0045651

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447775/
https://www.ncbi.nlm.nih.gov/pubmed/23029161
http://dx.doi.org/10.1371/journal.pone.0045651

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