Cargando…
Activation Biosensor for G Protein-Coupled Receptors: A FRET-Based m1 Muscarinic Activation Sensor That Regulates G(q)
We describe the design, construction and validation of a fluorescence sensor to measure activation by agonist of the m1 muscarinic cholinergic receptor, a prototypical class I G(q)-coupled receptor. The sensor uses an established general design in which Förster resonance energy transfer (FRET) from...
Autores principales: | Chang, Seungwoo, Ross, Elliott M. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447775/ https://www.ncbi.nlm.nih.gov/pubmed/23029161 http://dx.doi.org/10.1371/journal.pone.0045651 |
Ejemplares similares
-
Two-step structural changes in M3 muscarinic receptor activation rely on the coupled G(q) protein cycle
por: Kim, Yong-Seok, et al.
Publicado: (2023) -
The coupling of the M2 muscarinic receptor to its G protein is voltage dependent
por: Ben-Chaim, Yair, et al.
Publicado: (2019) -
FRET-Based Detection of M(1) Muscarinic Acetylcholine Receptor Activation by Orthosteric and Allosteric Agonists
por: Markovic, Danijela, et al.
Publicado: (2012) -
FRET sensors reveal the retinal entry pathway in the G protein-coupled receptor rhodopsin
por: Tian, He, et al.
Publicado: (2022) -
A FRET-based biosensor for measuring Gα13 activation in single cells
por: Mastop, Marieke, et al.
Publicado: (2018)