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A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine

The serum prostate-specific antigen (PSA) test has a high false positive rate. As a single marker, PSA provides limited diagnostic information. A multi-marker test capable of detecting not only tumors but also the potentially lethal ones provides an unmet clinical need. Using the nanoString nCounter...

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Autores principales: Quek, Sue-Ing, Ho, Melissa E., Loprieno, Michelle A., Ellis, William J., Elliott, Nathan, Liu, Alvin Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447789/
https://www.ncbi.nlm.nih.gov/pubmed/23029164
http://dx.doi.org/10.1371/journal.pone.0045656
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author Quek, Sue-Ing
Ho, Melissa E.
Loprieno, Michelle A.
Ellis, William J.
Elliott, Nathan
Liu, Alvin Y.
author_facet Quek, Sue-Ing
Ho, Melissa E.
Loprieno, Michelle A.
Ellis, William J.
Elliott, Nathan
Liu, Alvin Y.
author_sort Quek, Sue-Ing
collection PubMed
description The serum prostate-specific antigen (PSA) test has a high false positive rate. As a single marker, PSA provides limited diagnostic information. A multi-marker test capable of detecting not only tumors but also the potentially lethal ones provides an unmet clinical need. Using the nanoString nCounter gene expression system, a 20-gene multiplex test was developed based on digital gene counting of RNA transcripts in urine as a means to detect prostate cancer. In this test, voided urine is centrifuged to pellet cells and the purified RNA is amplified for hybridization to preselected probesets. Amplification of test cell line RNA appeared not to introduce significant bias, and the counts matched well with gene abundance levels as measured by DNA microarrays. For data analysis, the individual counts were compared to that of β2 microglobulin, a housekeeping gene. Urine samples of 5 pre-operative cases and 2 non-cancer were analyzed. Pathology information was then retrieved. Signals for a majority of the genes were low for non-cancer and low Gleason scores, and 6/6 known prostate cancer markers were positive in the cases. One case of Gleason 4+5 showed, in contrast, strong signals for all cancer-associated markers, including CD24. One non-cancer also showed signals for all 6 cancer markers, and this man might harbor an undiagnosed cancer. This multiplex test assaying a natural waste product can potentially be used for screening, early cancer detection and patient stratification. Diagnostic information is gained from the RNA signatures that are associated with cell types of prostate tumors.
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spelling pubmed-34477892012-10-01 A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine Quek, Sue-Ing Ho, Melissa E. Loprieno, Michelle A. Ellis, William J. Elliott, Nathan Liu, Alvin Y. PLoS One Research Article The serum prostate-specific antigen (PSA) test has a high false positive rate. As a single marker, PSA provides limited diagnostic information. A multi-marker test capable of detecting not only tumors but also the potentially lethal ones provides an unmet clinical need. Using the nanoString nCounter gene expression system, a 20-gene multiplex test was developed based on digital gene counting of RNA transcripts in urine as a means to detect prostate cancer. In this test, voided urine is centrifuged to pellet cells and the purified RNA is amplified for hybridization to preselected probesets. Amplification of test cell line RNA appeared not to introduce significant bias, and the counts matched well with gene abundance levels as measured by DNA microarrays. For data analysis, the individual counts were compared to that of β2 microglobulin, a housekeeping gene. Urine samples of 5 pre-operative cases and 2 non-cancer were analyzed. Pathology information was then retrieved. Signals for a majority of the genes were low for non-cancer and low Gleason scores, and 6/6 known prostate cancer markers were positive in the cases. One case of Gleason 4+5 showed, in contrast, strong signals for all cancer-associated markers, including CD24. One non-cancer also showed signals for all 6 cancer markers, and this man might harbor an undiagnosed cancer. This multiplex test assaying a natural waste product can potentially be used for screening, early cancer detection and patient stratification. Diagnostic information is gained from the RNA signatures that are associated with cell types of prostate tumors. Public Library of Science 2012-09-20 /pmc/articles/PMC3447789/ /pubmed/23029164 http://dx.doi.org/10.1371/journal.pone.0045656 Text en © 2012 Quek et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Quek, Sue-Ing
Ho, Melissa E.
Loprieno, Michelle A.
Ellis, William J.
Elliott, Nathan
Liu, Alvin Y.
A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title_full A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title_fullStr A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title_full_unstemmed A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title_short A Multiplex Assay to Measure RNA Transcripts of Prostate Cancer in Urine
title_sort multiplex assay to measure rna transcripts of prostate cancer in urine
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447789/
https://www.ncbi.nlm.nih.gov/pubmed/23029164
http://dx.doi.org/10.1371/journal.pone.0045656
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