Cargando…
Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2
Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in viv...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447859/ https://www.ncbi.nlm.nih.gov/pubmed/23028527 http://dx.doi.org/10.1371/journal.pone.0044351 |
_version_ | 1782244179848462336 |
---|---|
author | van Zoggel, Hanneke Carpentier, Gilles Dos Santos, Célia Hamma-Kourbali, Yamina Courty, José Amiche, Mohamed Delbé, Jean |
author_facet | van Zoggel, Hanneke Carpentier, Gilles Dos Santos, Célia Hamma-Kourbali, Yamina Courty, José Amiche, Mohamed Delbé, Jean |
author_sort | van Zoggel, Hanneke |
collection | PubMed |
description | Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in vivo antitumor activity of this molecule and investigated its mechanism of action. We showed that Drs B2 inhibits the proliferation and colony formation of various human tumor cell types, and the proliferation and capillary formation of endothelial cells in vitro. Furthermore, Drs B2 inhibited tumor growth of the human prostate adenocarcinoma cell line PC3 in a xenograft model in vivo. Research on the mechanism of action of Drs B2 on tumor PC3 cells demonstrated a rapid increasing amount of cytosolic lactate dehydrogenase, no activation of caspase-3, and no changes in mitochondrial membrane potential. Confocal microscopy analysis revealed that Drs B2 can interact with the tumor cell surface, aggregate and penetrate the cells. These data together indicate that Drs B2 does not act by apoptosis but possibly by necrosis. In conclusion, Drs B2 could be considered as an interesting and promising pharmacological and therapeutic leader molecule for the treatment of cancer. |
format | Online Article Text |
id | pubmed-3447859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34478592012-10-01 Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 van Zoggel, Hanneke Carpentier, Gilles Dos Santos, Célia Hamma-Kourbali, Yamina Courty, José Amiche, Mohamed Delbé, Jean PLoS One Research Article Recently, we have found that the skin secretions of the Amazonian tree frog Phyllomedusa bicolor contains molecules with antitumor and angiostatic activities and identified one of them as the antimicrobial peptide dermaseptin (Drs) B2. In the present study we further explored the in vitro and in vivo antitumor activity of this molecule and investigated its mechanism of action. We showed that Drs B2 inhibits the proliferation and colony formation of various human tumor cell types, and the proliferation and capillary formation of endothelial cells in vitro. Furthermore, Drs B2 inhibited tumor growth of the human prostate adenocarcinoma cell line PC3 in a xenograft model in vivo. Research on the mechanism of action of Drs B2 on tumor PC3 cells demonstrated a rapid increasing amount of cytosolic lactate dehydrogenase, no activation of caspase-3, and no changes in mitochondrial membrane potential. Confocal microscopy analysis revealed that Drs B2 can interact with the tumor cell surface, aggregate and penetrate the cells. These data together indicate that Drs B2 does not act by apoptosis but possibly by necrosis. In conclusion, Drs B2 could be considered as an interesting and promising pharmacological and therapeutic leader molecule for the treatment of cancer. Public Library of Science 2012-09-20 /pmc/articles/PMC3447859/ /pubmed/23028527 http://dx.doi.org/10.1371/journal.pone.0044351 Text en © 2012 van Zoggel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article van Zoggel, Hanneke Carpentier, Gilles Dos Santos, Célia Hamma-Kourbali, Yamina Courty, José Amiche, Mohamed Delbé, Jean Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title | Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title_full | Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title_fullStr | Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title_full_unstemmed | Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title_short | Antitumor and Angiostatic Activities of the Antimicrobial Peptide Dermaseptin B2 |
title_sort | antitumor and angiostatic activities of the antimicrobial peptide dermaseptin b2 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447859/ https://www.ncbi.nlm.nih.gov/pubmed/23028527 http://dx.doi.org/10.1371/journal.pone.0044351 |
work_keys_str_mv | AT vanzoggelhanneke antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT carpentiergilles antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT dossantoscelia antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT hammakourbaliyamina antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT courtyjose antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT amichemohamed antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 AT delbejean antitumorandangiostaticactivitiesoftheantimicrobialpeptidedermaseptinb2 |