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Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission

BACKGROUND: Giardia duodenalis is a widespread intestinal protozoan of both humans and mammals. To date, few epidemiological studies have assessed the potential and importance of zoonotic transmission; and the human giardiasis burden attributable to G. duodenalis of animal origin is unclear. No info...

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Autores principales: Zhang, Weizhe, Zhang, Xiaoli, Wang, Rongjun, Liu, Aiqin, Shen, Yujuan, Ling, Hong, Cao, Jianping, Yang, Fengkun, Zhang, Xiaoyun, Zhang, Longxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447956/
https://www.ncbi.nlm.nih.gov/pubmed/23029587
http://dx.doi.org/10.1371/journal.pntd.0001826
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author Zhang, Weizhe
Zhang, Xiaoli
Wang, Rongjun
Liu, Aiqin
Shen, Yujuan
Ling, Hong
Cao, Jianping
Yang, Fengkun
Zhang, Xiaoyun
Zhang, Longxian
author_facet Zhang, Weizhe
Zhang, Xiaoli
Wang, Rongjun
Liu, Aiqin
Shen, Yujuan
Ling, Hong
Cao, Jianping
Yang, Fengkun
Zhang, Xiaoyun
Zhang, Longxian
author_sort Zhang, Weizhe
collection PubMed
description BACKGROUND: Giardia duodenalis is a widespread intestinal protozoan of both humans and mammals. To date, few epidemiological studies have assessed the potential and importance of zoonotic transmission; and the human giardiasis burden attributable to G. duodenalis of animal origin is unclear. No information about occurrence and genotyping data of sheep and goat giardiasis is available in China. The aim of the present study was to determine prevalence and distribution of G. duodenalis in sheep and goats in Heilongjiang Province, China, and to characterize G. duodenalis isolates and assess the possibility of zoonotic transmission. METHODOLOGY/PRINCIPAL FINDINGS: A total of 678 fecal specimens were collected from sheep and goats on six farms ranging in age from one month to four years in Heilongjiang Province, China. The average prevalence of G. duodenalis infection was 5.0% (34/678) by microscopy after Lugol's iodine staining, with 5.6% (30/539) for the sheep versus 2.9% (4/139) for the goats. Molecular analysis was conducted on 34 G. duodenalis isolates based on the triosephosphate isomerase (tpi) gene. 29 tpi gene sequences were successfully obtained and identified as assemblages A (n = 4), B (n = 2) and E (n = 23). High heterogeneity was observed within assemblage E at the tpi locus, with five novel subtypes found out of seven subtypes. Two subtypes of assemblage A were detected, including subtype AI (n = 3) and a novel subtype (designated as subtype AIV) (n = 1). Two assemblage B isolates were identical to each other in the tpi gene sequences. CONCLUSIONS/SIGNIFICANCE: This is the first report of G. duodenalis infections in sheep and goats in China. The present data revealed the unique endemicity on prevalence, distribution and genetic characterization of G. duodenalis in sheep and goats in Heilongjiang Province. The findings of assemblages A and B in sheep and goats implied the potential of zoonotic transmission.
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spelling pubmed-34479562012-10-01 Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission Zhang, Weizhe Zhang, Xiaoli Wang, Rongjun Liu, Aiqin Shen, Yujuan Ling, Hong Cao, Jianping Yang, Fengkun Zhang, Xiaoyun Zhang, Longxian PLoS Negl Trop Dis Research Article BACKGROUND: Giardia duodenalis is a widespread intestinal protozoan of both humans and mammals. To date, few epidemiological studies have assessed the potential and importance of zoonotic transmission; and the human giardiasis burden attributable to G. duodenalis of animal origin is unclear. No information about occurrence and genotyping data of sheep and goat giardiasis is available in China. The aim of the present study was to determine prevalence and distribution of G. duodenalis in sheep and goats in Heilongjiang Province, China, and to characterize G. duodenalis isolates and assess the possibility of zoonotic transmission. METHODOLOGY/PRINCIPAL FINDINGS: A total of 678 fecal specimens were collected from sheep and goats on six farms ranging in age from one month to four years in Heilongjiang Province, China. The average prevalence of G. duodenalis infection was 5.0% (34/678) by microscopy after Lugol's iodine staining, with 5.6% (30/539) for the sheep versus 2.9% (4/139) for the goats. Molecular analysis was conducted on 34 G. duodenalis isolates based on the triosephosphate isomerase (tpi) gene. 29 tpi gene sequences were successfully obtained and identified as assemblages A (n = 4), B (n = 2) and E (n = 23). High heterogeneity was observed within assemblage E at the tpi locus, with five novel subtypes found out of seven subtypes. Two subtypes of assemblage A were detected, including subtype AI (n = 3) and a novel subtype (designated as subtype AIV) (n = 1). Two assemblage B isolates were identical to each other in the tpi gene sequences. CONCLUSIONS/SIGNIFICANCE: This is the first report of G. duodenalis infections in sheep and goats in China. The present data revealed the unique endemicity on prevalence, distribution and genetic characterization of G. duodenalis in sheep and goats in Heilongjiang Province. The findings of assemblages A and B in sheep and goats implied the potential of zoonotic transmission. Public Library of Science 2012-09-20 /pmc/articles/PMC3447956/ /pubmed/23029587 http://dx.doi.org/10.1371/journal.pntd.0001826 Text en © 2012 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Weizhe
Zhang, Xiaoli
Wang, Rongjun
Liu, Aiqin
Shen, Yujuan
Ling, Hong
Cao, Jianping
Yang, Fengkun
Zhang, Xiaoyun
Zhang, Longxian
Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title_full Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title_fullStr Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title_full_unstemmed Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title_short Genetic Characterizations of Giardia duodenalis in Sheep and Goats in Heilongjiang Province, China and Possibility of Zoonotic Transmission
title_sort genetic characterizations of giardia duodenalis in sheep and goats in heilongjiang province, china and possibility of zoonotic transmission
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3447956/
https://www.ncbi.nlm.nih.gov/pubmed/23029587
http://dx.doi.org/10.1371/journal.pntd.0001826
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