Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin

The expression of CD10 has long been used to define human lymphoid commitment. We report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before the onset of CD10 expression and B cell commitment. This subset was identified by high expr...

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Autores principales: Kohn, Lisa A., Hao, Qian-Lin, Sasidharan, Rajkumar, Parekh, Chintan, Ge, Shundi, Zhu, Yuhua, Mikkola, Hanna K.A., Crooks, Gay M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448017/
https://www.ncbi.nlm.nih.gov/pubmed/22941246
http://dx.doi.org/10.1038/ni.2405
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author Kohn, Lisa A.
Hao, Qian-Lin
Sasidharan, Rajkumar
Parekh, Chintan
Ge, Shundi
Zhu, Yuhua
Mikkola, Hanna K.A.
Crooks, Gay M.
author_facet Kohn, Lisa A.
Hao, Qian-Lin
Sasidharan, Rajkumar
Parekh, Chintan
Ge, Shundi
Zhu, Yuhua
Mikkola, Hanna K.A.
Crooks, Gay M.
author_sort Kohn, Lisa A.
collection PubMed
description The expression of CD10 has long been used to define human lymphoid commitment. We report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before the onset of CD10 expression and B cell commitment. This subset was identified by high expression of the homing molecule L-selectin (CD62L). CD10(−)CD62L(hi) progenitors possessed full lymphoid and monocytic potential, but lacked erythroid potential. Gene expression profiling placed the CD10(−)CD62L(hi) population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin(−)) CD34(+)CD10(+) progenitors. L-selectin was expressed on immature thymocytes and its ligands were expressed at the cortico-medullary junction, suggesting a possible role in thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow.
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spelling pubmed-34480172013-04-01 Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin Kohn, Lisa A. Hao, Qian-Lin Sasidharan, Rajkumar Parekh, Chintan Ge, Shundi Zhu, Yuhua Mikkola, Hanna K.A. Crooks, Gay M. Nat Immunol Article The expression of CD10 has long been used to define human lymphoid commitment. We report a unique lymphoid-primed population in human bone marrow that was generated from hematopoietic stem cells (HSCs) before the onset of CD10 expression and B cell commitment. This subset was identified by high expression of the homing molecule L-selectin (CD62L). CD10(−)CD62L(hi) progenitors possessed full lymphoid and monocytic potential, but lacked erythroid potential. Gene expression profiling placed the CD10(−)CD62L(hi) population at an intermediate stage of differentiation between HSCs and lineage-negative (Lin(−)) CD34(+)CD10(+) progenitors. L-selectin was expressed on immature thymocytes and its ligands were expressed at the cortico-medullary junction, suggesting a possible role in thymic homing. These studies identify the earliest stage of lymphoid priming in human bone marrow. 2012-09-02 2012-10 /pmc/articles/PMC3448017/ /pubmed/22941246 http://dx.doi.org/10.1038/ni.2405 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kohn, Lisa A.
Hao, Qian-Lin
Sasidharan, Rajkumar
Parekh, Chintan
Ge, Shundi
Zhu, Yuhua
Mikkola, Hanna K.A.
Crooks, Gay M.
Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title_full Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title_fullStr Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title_full_unstemmed Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title_short Lymphoid Priming in Human Bone Marrow Begins Prior to CD10 Expression with Up-Regulation of L-selectin
title_sort lymphoid priming in human bone marrow begins prior to cd10 expression with up-regulation of l-selectin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448017/
https://www.ncbi.nlm.nih.gov/pubmed/22941246
http://dx.doi.org/10.1038/ni.2405
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