Cargando…

Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)

Bacterial endophytes are crucial for the survival of many terrestrial plants, but little is known about the presence and importance of bacterial endophytes of marine plants. We conducted a survey of the endophytic bacterial community of the long-living Mediterranean marine angiosperm Posidonia ocean...

Descripción completa

Detalles Bibliográficos
Autores principales: Garcias-Bonet, Neus, Arrieta, Jesus M., de Santana, Charles N., Duarte, Carlos M., Marbà, Núria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448135/
https://www.ncbi.nlm.nih.gov/pubmed/23049528
http://dx.doi.org/10.3389/fmicb.2012.00342
_version_ 1782244223302500352
author Garcias-Bonet, Neus
Arrieta, Jesus M.
de Santana, Charles N.
Duarte, Carlos M.
Marbà, Núria
author_facet Garcias-Bonet, Neus
Arrieta, Jesus M.
de Santana, Charles N.
Duarte, Carlos M.
Marbà, Núria
author_sort Garcias-Bonet, Neus
collection PubMed
description Bacterial endophytes are crucial for the survival of many terrestrial plants, but little is known about the presence and importance of bacterial endophytes of marine plants. We conducted a survey of the endophytic bacterial community of the long-living Mediterranean marine angiosperm Posidonia oceanica in surface-sterilized tissues (roots, rhizomes, and leaves) by Denaturing Gradient Gel Electrophoresis (DGGE). A total of 26 Posidonia oceanica meadows around the Balearic Islands were sampled, and the band patterns obtained for each meadow were compared for the three sampled tissues. Endophytic bacterial sequences were detected in most of the samples analyzed. A total of 34 OTUs (Operational Taxonomic Units) were detected. The main OTUs of endophytic bacteria present in P. oceanica tissues belonged primarily to Proteobacteria (α, γ, and δ subclasses) and Bacteroidetes. The OTUs found in roots significantly differed from those of rhizomes and leaves. Moreover, some OTUs were found to be associated to each type of tissue. Bipartite network analysis revealed differences in the bacterial endophyte communities present on different islands. The results of this study provide a pioneering step toward the characterization of the endophytic bacterial community associated with tissues of a marine angiosperm and reveal the presence of bacterial endophytes that differed among locations and tissue types.
format Online
Article
Text
id pubmed-3448135
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-34481352012-10-04 Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica) Garcias-Bonet, Neus Arrieta, Jesus M. de Santana, Charles N. Duarte, Carlos M. Marbà, Núria Front Microbiol Microbiology Bacterial endophytes are crucial for the survival of many terrestrial plants, but little is known about the presence and importance of bacterial endophytes of marine plants. We conducted a survey of the endophytic bacterial community of the long-living Mediterranean marine angiosperm Posidonia oceanica in surface-sterilized tissues (roots, rhizomes, and leaves) by Denaturing Gradient Gel Electrophoresis (DGGE). A total of 26 Posidonia oceanica meadows around the Balearic Islands were sampled, and the band patterns obtained for each meadow were compared for the three sampled tissues. Endophytic bacterial sequences were detected in most of the samples analyzed. A total of 34 OTUs (Operational Taxonomic Units) were detected. The main OTUs of endophytic bacteria present in P. oceanica tissues belonged primarily to Proteobacteria (α, γ, and δ subclasses) and Bacteroidetes. The OTUs found in roots significantly differed from those of rhizomes and leaves. Moreover, some OTUs were found to be associated to each type of tissue. Bipartite network analysis revealed differences in the bacterial endophyte communities present on different islands. The results of this study provide a pioneering step toward the characterization of the endophytic bacterial community associated with tissues of a marine angiosperm and reveal the presence of bacterial endophytes that differed among locations and tissue types. Frontiers Media S.A. 2012-09-21 /pmc/articles/PMC3448135/ /pubmed/23049528 http://dx.doi.org/10.3389/fmicb.2012.00342 Text en Copyright © 2012 Garcias-Bonet, Arrieta, de Santana, Duarte and Marbà. http://www.frontiersin.org/licenseagreement This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Microbiology
Garcias-Bonet, Neus
Arrieta, Jesus M.
de Santana, Charles N.
Duarte, Carlos M.
Marbà, Núria
Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title_full Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title_fullStr Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title_full_unstemmed Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title_short Endophytic bacterial community of a Mediterranean marine angiosperm (Posidonia oceanica)
title_sort endophytic bacterial community of a mediterranean marine angiosperm (posidonia oceanica)
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448135/
https://www.ncbi.nlm.nih.gov/pubmed/23049528
http://dx.doi.org/10.3389/fmicb.2012.00342
work_keys_str_mv AT garciasbonetneus endophyticbacterialcommunityofamediterraneanmarineangiospermposidoniaoceanica
AT arrietajesusm endophyticbacterialcommunityofamediterraneanmarineangiospermposidoniaoceanica
AT desantanacharlesn endophyticbacterialcommunityofamediterraneanmarineangiospermposidoniaoceanica
AT duartecarlosm endophyticbacterialcommunityofamediterraneanmarineangiospermposidoniaoceanica
AT marbanuria endophyticbacterialcommunityofamediterraneanmarineangiospermposidoniaoceanica