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Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent
Objectives: Orofacial clefts are major human birth defects with complex etiology. Previous studies have proposed Transforming growth factor - beta 3 (TGF-β3) gene as a key player in contributing to non-syndromic cleft lip and palate, however none of the studies have yet included Indian population. H...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medicina Oral S.L.
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448322/ https://www.ncbi.nlm.nih.gov/pubmed/22143699 http://dx.doi.org/10.4317/medoral.17453 |
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author | Shaikh, Saleem Ravenndranath, Rajendran Banerjee, Moinak Joseph, Anna Jahgirdar, Pramod |
author_facet | Shaikh, Saleem Ravenndranath, Rajendran Banerjee, Moinak Joseph, Anna Jahgirdar, Pramod |
author_sort | Shaikh, Saleem |
collection | PubMed |
description | Objectives: Orofacial clefts are major human birth defects with complex etiology. Previous studies have proposed Transforming growth factor - beta 3 (TGF-β3) gene as a key player in contributing to non-syndromic cleft lip and palate, however none of the studies have yet included Indian population. Hence this study was designed to detect TGF-β3 gene polymorphism in nonsyndromic cleft lip and palate patients from Indian population which is genetically distinct from previously studied populations. Study Design: Peripheral blood samples of forty non-syndromic cleft lip and palate patients and forty unaffected individuals were collected for a case – control study design. Ethical clearance from the institutional review board and informed consent from all subjects was obtained. DNA extracted from the cases and controls was amplified using polymerase chain reaction (PCR) with TGF-β3 specific primers. The obtained fragments were sequenced and TGF-β3 gene polymorphisms were assessed based on the number of CA repeats. Results: Chi –square test was used to compare the case and control groups. Results showed a significant difference in the number of CA repeats between the case and the control groups (p=0.01). Conclusion: This study confirms the crucial role of TGF-β3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-β3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation. Key words: Orofacial clefts, nonsyndromic cleft lip with/without cleft palate, TGF-β3, Polymerase chain reaction, gene polymorphism. |
format | Online Article Text |
id | pubmed-3448322 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Medicina Oral S.L. |
record_format | MEDLINE/PubMed |
spelling | pubmed-34483222012-10-11 Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent Shaikh, Saleem Ravenndranath, Rajendran Banerjee, Moinak Joseph, Anna Jahgirdar, Pramod Med Oral Patol Oral Cir Bucal Research-Article Objectives: Orofacial clefts are major human birth defects with complex etiology. Previous studies have proposed Transforming growth factor - beta 3 (TGF-β3) gene as a key player in contributing to non-syndromic cleft lip and palate, however none of the studies have yet included Indian population. Hence this study was designed to detect TGF-β3 gene polymorphism in nonsyndromic cleft lip and palate patients from Indian population which is genetically distinct from previously studied populations. Study Design: Peripheral blood samples of forty non-syndromic cleft lip and palate patients and forty unaffected individuals were collected for a case – control study design. Ethical clearance from the institutional review board and informed consent from all subjects was obtained. DNA extracted from the cases and controls was amplified using polymerase chain reaction (PCR) with TGF-β3 specific primers. The obtained fragments were sequenced and TGF-β3 gene polymorphisms were assessed based on the number of CA repeats. Results: Chi –square test was used to compare the case and control groups. Results showed a significant difference in the number of CA repeats between the case and the control groups (p=0.01). Conclusion: This study confirms the crucial role of TGF-β3 in the fusion of palatal shelves during development and further, provides novel evidence of TGF-β3 gene polymorphism in the etiology of nonsyndromic cleft lip and palate in Indian subpopulation. Key words: Orofacial clefts, nonsyndromic cleft lip with/without cleft palate, TGF-β3, Polymerase chain reaction, gene polymorphism. Medicina Oral S.L. 2012-03 2011-12-06 /pmc/articles/PMC3448322/ /pubmed/22143699 http://dx.doi.org/10.4317/medoral.17453 Text en Copyright: © 2012 Medicina Oral S.L. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research-Article Shaikh, Saleem Ravenndranath, Rajendran Banerjee, Moinak Joseph, Anna Jahgirdar, Pramod Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title | Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title_full | Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title_fullStr | Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title_full_unstemmed | Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title_short | Evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
title_sort | evidence for transforming growth factor–beta 3 gene polymorphism in non-syndromic cleft lip and palate patients from indian sub-continent |
topic | Research-Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448322/ https://www.ncbi.nlm.nih.gov/pubmed/22143699 http://dx.doi.org/10.4317/medoral.17453 |
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