Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software...

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Detalles Bibliográficos
Autores principales: Siritantikorn, Sontana, Jintaworn, Suthatip, Noisakran, Sansanee, Suputtamongkol, Yupin, Paris, Daniel H., Blacksell, Stuart D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449237/
https://www.ncbi.nlm.nih.gov/pubmed/22789674
http://dx.doi.org/10.1016/j.trstmh.2012.05.004
Descripción
Sumario:The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.

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