Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro

The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software...

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Detalles Bibliográficos
Autores principales: Siritantikorn, Sontana, Jintaworn, Suthatip, Noisakran, Sansanee, Suputtamongkol, Yupin, Paris, Daniel H., Blacksell, Stuart D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449237/
https://www.ncbi.nlm.nih.gov/pubmed/22789674
http://dx.doi.org/10.1016/j.trstmh.2012.05.004
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author Siritantikorn, Sontana
Jintaworn, Suthatip
Noisakran, Sansanee
Suputtamongkol, Yupin
Paris, Daniel H.
Blacksell, Stuart D.
author_facet Siritantikorn, Sontana
Jintaworn, Suthatip
Noisakran, Sansanee
Suputtamongkol, Yupin
Paris, Daniel H.
Blacksell, Stuart D.
author_sort Siritantikorn, Sontana
collection PubMed
description The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach.
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spelling pubmed-34492372012-10-01 Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro Siritantikorn, Sontana Jintaworn, Suthatip Noisakran, Sansanee Suputtamongkol, Yupin Paris, Daniel H. Blacksell, Stuart D. Trans R Soc Trop Med Hyg Short Communication The ImageJ program was applied to the enumeration of Orientia tsutsugamushi organisms in cell culture using indirect immunofluorescence assay (IFA). The highest correlation (r = 0.984) was observed between manual counting methods and the ImageJ program (MaxEntropy threshold algorithm). This software-based methodology is cheaper, more standardised and better reproducible than a manual-based approach. Oxford University Press 2012-10 /pmc/articles/PMC3449237/ /pubmed/22789674 http://dx.doi.org/10.1016/j.trstmh.2012.05.004 Text en © 2012 Elsevier Ltd. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Short Communication
Siritantikorn, Sontana
Jintaworn, Suthatip
Noisakran, Sansanee
Suputtamongkol, Yupin
Paris, Daniel H.
Blacksell, Stuart D.
Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title_full Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title_fullStr Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title_full_unstemmed Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title_short Application of ImageJ program to the enumeration of Orientia tsutsugamushi organisms cultured in vitro
title_sort application of imagej program to the enumeration of orientia tsutsugamushi organisms cultured in vitro
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3449237/
https://www.ncbi.nlm.nih.gov/pubmed/22789674
http://dx.doi.org/10.1016/j.trstmh.2012.05.004
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