Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale

Emerging questions in cell biology necessitate nanometer-scale imaging in live cells. Here we present scanning angle interference microscopy, capable of localizing fluorescent objects with nanometer-scale precision along the optical axis in motile cellular structures. We use this approach to resolve...

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Detalles Bibliográficos
Autores principales: Paszek, Matthew J., DuFort, Christopher C., Rubashkin, Matthew G., Davidson, Mike W., Thorn, Kurt S., Liphardt, Jan T., Weaver, Valerie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454456/
https://www.ncbi.nlm.nih.gov/pubmed/22751201
http://dx.doi.org/10.1038/nmeth.2077
Descripción
Sumario:Emerging questions in cell biology necessitate nanometer-scale imaging in live cells. Here we present scanning angle interference microscopy, capable of localizing fluorescent objects with nanometer-scale precision along the optical axis in motile cellular structures. We use this approach to resolve nano-topographical features of the cell membrane and cytoskeleton, as well as the temporal evolution, three-dimensional architecture, and nano-scale dynamics of focal adhesion complexes.

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