Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale

Emerging questions in cell biology necessitate nanometer-scale imaging in live cells. Here we present scanning angle interference microscopy, capable of localizing fluorescent objects with nanometer-scale precision along the optical axis in motile cellular structures. We use this approach to resolve...

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Detalles Bibliográficos
Autores principales: Paszek, Matthew J., DuFort, Christopher C., Rubashkin, Matthew G., Davidson, Mike W., Thorn, Kurt S., Liphardt, Jan T., Weaver, Valerie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454456/
https://www.ncbi.nlm.nih.gov/pubmed/22751201
http://dx.doi.org/10.1038/nmeth.2077
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author Paszek, Matthew J.
DuFort, Christopher C.
Rubashkin, Matthew G.
Davidson, Mike W.
Thorn, Kurt S.
Liphardt, Jan T.
Weaver, Valerie M.
author_facet Paszek, Matthew J.
DuFort, Christopher C.
Rubashkin, Matthew G.
Davidson, Mike W.
Thorn, Kurt S.
Liphardt, Jan T.
Weaver, Valerie M.
author_sort Paszek, Matthew J.
collection PubMed
description Emerging questions in cell biology necessitate nanometer-scale imaging in live cells. Here we present scanning angle interference microscopy, capable of localizing fluorescent objects with nanometer-scale precision along the optical axis in motile cellular structures. We use this approach to resolve nano-topographical features of the cell membrane and cytoskeleton, as well as the temporal evolution, three-dimensional architecture, and nano-scale dynamics of focal adhesion complexes.
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spelling pubmed-34544562013-02-01 Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale Paszek, Matthew J. DuFort, Christopher C. Rubashkin, Matthew G. Davidson, Mike W. Thorn, Kurt S. Liphardt, Jan T. Weaver, Valerie M. Nat Methods Article Emerging questions in cell biology necessitate nanometer-scale imaging in live cells. Here we present scanning angle interference microscopy, capable of localizing fluorescent objects with nanometer-scale precision along the optical axis in motile cellular structures. We use this approach to resolve nano-topographical features of the cell membrane and cytoskeleton, as well as the temporal evolution, three-dimensional architecture, and nano-scale dynamics of focal adhesion complexes. 2012-07-01 /pmc/articles/PMC3454456/ /pubmed/22751201 http://dx.doi.org/10.1038/nmeth.2077 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Paszek, Matthew J.
DuFort, Christopher C.
Rubashkin, Matthew G.
Davidson, Mike W.
Thorn, Kurt S.
Liphardt, Jan T.
Weaver, Valerie M.
Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title_full Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title_fullStr Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title_full_unstemmed Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title_short Scanning Angle Interference Microscopy Reveals Cell Dynamics at the Nano-scale
title_sort scanning angle interference microscopy reveals cell dynamics at the nano-scale
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454456/
https://www.ncbi.nlm.nih.gov/pubmed/22751201
http://dx.doi.org/10.1038/nmeth.2077
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