Cargando…

The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis

Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found t...

Descripción completa

Detalles Bibliográficos
Autores principales: Hsiao, Chia-Yen, Tsai, Tung-Hu, Chak, Kin-Fu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457683/
https://www.ncbi.nlm.nih.gov/pubmed/23024692
http://dx.doi.org/10.1155/2012/508972
_version_ 1782244555641323520
author Hsiao, Chia-Yen
Tsai, Tung-Hu
Chak, Kin-Fu
author_facet Hsiao, Chia-Yen
Tsai, Tung-Hu
Chak, Kin-Fu
author_sort Hsiao, Chia-Yen
collection PubMed
description Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found that both LR ethanol extracts and shikonin are able to promote cell proliferation by up to 25%. The results of proteomic analysis revealed that twenty-two differentially expressed proteins could be identified when fibroblast cells were treated with LR or shikonin. The functions of those proteins are associated with antioxidant activity, antiapoptosis activity, the regulation of cell mobility, the secretion of collagen, the removal of abnormal proteins, and the promotion of cell proliferation, indicating that the efficacy of LR in wound healing may be derived from a synergistic effect on a number of factors induced by the herbal medicine. Furthermore, an animal model confirmed that LR is able to accelerate wound healing on the flank back of the SD rats. Together these findings help to pinpoint the molecular basis of wound healing process induced by LR.
format Online
Article
Text
id pubmed-3457683
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-34576832012-09-28 The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis Hsiao, Chia-Yen Tsai, Tung-Hu Chak, Kin-Fu Evid Based Complement Alternat Med Research Article Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found that both LR ethanol extracts and shikonin are able to promote cell proliferation by up to 25%. The results of proteomic analysis revealed that twenty-two differentially expressed proteins could be identified when fibroblast cells were treated with LR or shikonin. The functions of those proteins are associated with antioxidant activity, antiapoptosis activity, the regulation of cell mobility, the secretion of collagen, the removal of abnormal proteins, and the promotion of cell proliferation, indicating that the efficacy of LR in wound healing may be derived from a synergistic effect on a number of factors induced by the herbal medicine. Furthermore, an animal model confirmed that LR is able to accelerate wound healing on the flank back of the SD rats. Together these findings help to pinpoint the molecular basis of wound healing process induced by LR. Hindawi Publishing Corporation 2012 2012-09-17 /pmc/articles/PMC3457683/ /pubmed/23024692 http://dx.doi.org/10.1155/2012/508972 Text en Copyright © 2012 Chia-Yen Hsiao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Hsiao, Chia-Yen
Tsai, Tung-Hu
Chak, Kin-Fu
The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title_full The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title_fullStr The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title_full_unstemmed The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title_short The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
title_sort molecular basis of wound healing processes induced by lithospermi radix: a proteomics and biochemical analysis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457683/
https://www.ncbi.nlm.nih.gov/pubmed/23024692
http://dx.doi.org/10.1155/2012/508972
work_keys_str_mv AT hsiaochiayen themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis
AT tsaitunghu themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis
AT chakkinfu themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis
AT hsiaochiayen molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis
AT tsaitunghu molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis
AT chakkinfu molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis