Cargando…
The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis
Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found t...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457683/ https://www.ncbi.nlm.nih.gov/pubmed/23024692 http://dx.doi.org/10.1155/2012/508972 |
_version_ | 1782244555641323520 |
---|---|
author | Hsiao, Chia-Yen Tsai, Tung-Hu Chak, Kin-Fu |
author_facet | Hsiao, Chia-Yen Tsai, Tung-Hu Chak, Kin-Fu |
author_sort | Hsiao, Chia-Yen |
collection | PubMed |
description | Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found that both LR ethanol extracts and shikonin are able to promote cell proliferation by up to 25%. The results of proteomic analysis revealed that twenty-two differentially expressed proteins could be identified when fibroblast cells were treated with LR or shikonin. The functions of those proteins are associated with antioxidant activity, antiapoptosis activity, the regulation of cell mobility, the secretion of collagen, the removal of abnormal proteins, and the promotion of cell proliferation, indicating that the efficacy of LR in wound healing may be derived from a synergistic effect on a number of factors induced by the herbal medicine. Furthermore, an animal model confirmed that LR is able to accelerate wound healing on the flank back of the SD rats. Together these findings help to pinpoint the molecular basis of wound healing process induced by LR. |
format | Online Article Text |
id | pubmed-3457683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34576832012-09-28 The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis Hsiao, Chia-Yen Tsai, Tung-Hu Chak, Kin-Fu Evid Based Complement Alternat Med Research Article Lithospermi Radix (LR) is an effective traditional Chinese herb in various types of wound healing; however, its mechanism of action remains unknown. A biochemical and proteomic platform was generated to explore the biological phenomena associated with LR and its active component shikonin. We found that both LR ethanol extracts and shikonin are able to promote cell proliferation by up to 25%. The results of proteomic analysis revealed that twenty-two differentially expressed proteins could be identified when fibroblast cells were treated with LR or shikonin. The functions of those proteins are associated with antioxidant activity, antiapoptosis activity, the regulation of cell mobility, the secretion of collagen, the removal of abnormal proteins, and the promotion of cell proliferation, indicating that the efficacy of LR in wound healing may be derived from a synergistic effect on a number of factors induced by the herbal medicine. Furthermore, an animal model confirmed that LR is able to accelerate wound healing on the flank back of the SD rats. Together these findings help to pinpoint the molecular basis of wound healing process induced by LR. Hindawi Publishing Corporation 2012 2012-09-17 /pmc/articles/PMC3457683/ /pubmed/23024692 http://dx.doi.org/10.1155/2012/508972 Text en Copyright © 2012 Chia-Yen Hsiao et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hsiao, Chia-Yen Tsai, Tung-Hu Chak, Kin-Fu The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title | The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title_full | The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title_fullStr | The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title_full_unstemmed | The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title_short | The Molecular Basis of Wound Healing Processes Induced by Lithospermi Radix: A Proteomics and Biochemical Analysis |
title_sort | molecular basis of wound healing processes induced by lithospermi radix: a proteomics and biochemical analysis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457683/ https://www.ncbi.nlm.nih.gov/pubmed/23024692 http://dx.doi.org/10.1155/2012/508972 |
work_keys_str_mv | AT hsiaochiayen themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis AT tsaitunghu themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis AT chakkinfu themolecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis AT hsiaochiayen molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis AT tsaitunghu molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis AT chakkinfu molecularbasisofwoundhealingprocessesinducedbylithospermiradixaproteomicsandbiochemicalanalysis |