Macrophages induce differentiation of plasma cells through CXCL10/IP-10

In tonsils, CD138(+) plasma cells (PCs) are surrounded by CD163(+) resident macrophages (Mϕs). We show here that human Mϕs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138(+)CD38(++) PCs through secreted CXCL10/IP-10 and VCAM-1 contact....

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Autores principales: Xu, Wei, Joo, HyeMee, Clayton, Sandra, Dullaers, Melissa, Herve, Marie-Cecile, Blankenship, Derek, De La Morena, Maria Teresa, Balderas, Robert, Picard, Capucine, Casanova, Jean-Laurent, Pascual, Virginia, Oh, SangKon, Banchereau, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457728/
https://www.ncbi.nlm.nih.gov/pubmed/22987802
http://dx.doi.org/10.1084/jem.20112142
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author Xu, Wei
Joo, HyeMee
Clayton, Sandra
Dullaers, Melissa
Herve, Marie-Cecile
Blankenship, Derek
De La Morena, Maria Teresa
Balderas, Robert
Picard, Capucine
Casanova, Jean-Laurent
Pascual, Virginia
Oh, SangKon
Banchereau, Jacques
author_facet Xu, Wei
Joo, HyeMee
Clayton, Sandra
Dullaers, Melissa
Herve, Marie-Cecile
Blankenship, Derek
De La Morena, Maria Teresa
Balderas, Robert
Picard, Capucine
Casanova, Jean-Laurent
Pascual, Virginia
Oh, SangKon
Banchereau, Jacques
author_sort Xu, Wei
collection PubMed
description In tonsils, CD138(+) plasma cells (PCs) are surrounded by CD163(+) resident macrophages (Mϕs). We show here that human Mϕs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138(+)CD38(++) PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mϕs is induced by B cell–derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10–deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mϕs and B cells, in which IP-10 acts as a PC differentiation factor.
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spelling pubmed-34577282013-03-24 Macrophages induce differentiation of plasma cells through CXCL10/IP-10 Xu, Wei Joo, HyeMee Clayton, Sandra Dullaers, Melissa Herve, Marie-Cecile Blankenship, Derek De La Morena, Maria Teresa Balderas, Robert Picard, Capucine Casanova, Jean-Laurent Pascual, Virginia Oh, SangKon Banchereau, Jacques J Exp Med Article In tonsils, CD138(+) plasma cells (PCs) are surrounded by CD163(+) resident macrophages (Mϕs). We show here that human Mϕs (isolated from tonsils or generated from monocytes in vitro) drive activated B cells to differentiate into CD138(+)CD38(++) PCs through secreted CXCL10/IP-10 and VCAM-1 contact. IP-10 production by Mϕs is induced by B cell–derived IL-6 and depends on STAT3 phosphorylation. Furthermore, IP-10 amplifies the production of IL-6 by B cells, which sustains the STAT3 signals that lead to PC differentiation. IP-10–deficient mice challenged with NP-Ficoll show a decreased frequency of NP-specific PCs and lower titers of antibodies. Thus, our results reveal a novel dialog between Mϕs and B cells, in which IP-10 acts as a PC differentiation factor. The Rockefeller University Press 2012-09-24 /pmc/articles/PMC3457728/ /pubmed/22987802 http://dx.doi.org/10.1084/jem.20112142 Text en © 2012 Xu et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Article
Xu, Wei
Joo, HyeMee
Clayton, Sandra
Dullaers, Melissa
Herve, Marie-Cecile
Blankenship, Derek
De La Morena, Maria Teresa
Balderas, Robert
Picard, Capucine
Casanova, Jean-Laurent
Pascual, Virginia
Oh, SangKon
Banchereau, Jacques
Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title_full Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title_fullStr Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title_full_unstemmed Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title_short Macrophages induce differentiation of plasma cells through CXCL10/IP-10
title_sort macrophages induce differentiation of plasma cells through cxcl10/ip-10
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3457728/
https://www.ncbi.nlm.nih.gov/pubmed/22987802
http://dx.doi.org/10.1084/jem.20112142
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