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R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mou...

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Detalles Bibliográficos
Autores principales: Chen, You-Tzung, Tsai, Ming-Shian, Yang, Tsung-Lin, Ku, Amy Tsu, Huang, Ke-Han, Huang, Cheng-Yen, Chou, Fu-Ju, Fan, Hsiang-Hsuan, Hong, Jin-Bon, Yen, Shuo-Ting, Wang, Wei-Le, Lin, Chang-Ching, Hsu, Yu-Chen, Su, Kang-Yi, Su, I-Chang, Jang, Chuan-Wei, Behringer, Richard R., Favaro, Rebecca, Nicolis, Silvia K., Chien, Chung-Liang, Lin, Shu-Wha, Yu, I-Shing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458011/
https://www.ncbi.nlm.nih.gov/pubmed/23049968
http://dx.doi.org/10.1371/journal.pone.0046171
Descripción
Sumario:Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.