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Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice

OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2)(*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2)(*). We aimed to quantify T (2)(*) in acute and chronic myocardial ischemia/reperfusi...

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Autores principales: Aguor, Eissa N. E., Arslan, Fatih, van de Kolk, Cees W. A., Nederhoff, Marcel G. J., Doevendans, Pieter A., van Echteld, Cees J. A., Pasterkamp, Gerard, Strijkers, Gustav J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458196/
https://www.ncbi.nlm.nih.gov/pubmed/22327962
http://dx.doi.org/10.1007/s10334-012-0304-0
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author Aguor, Eissa N. E.
Arslan, Fatih
van de Kolk, Cees W. A.
Nederhoff, Marcel G. J.
Doevendans, Pieter A.
van Echteld, Cees J. A.
Pasterkamp, Gerard
Strijkers, Gustav J.
author_facet Aguor, Eissa N. E.
Arslan, Fatih
van de Kolk, Cees W. A.
Nederhoff, Marcel G. J.
Doevendans, Pieter A.
van Echteld, Cees J. A.
Pasterkamp, Gerard
Strijkers, Gustav J.
author_sort Aguor, Eissa N. E.
collection PubMed
description OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2)(*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2)(*). We aimed to quantify T (2)(*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice. MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2)(*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar. RESULTS: Baseline T (2)(*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2)(*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2)(*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2)(*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2)(*). CONCLUSION: T (2)(*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2)(*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2)(*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2)(*) may be used to discriminate between acute and chronic infarctions.
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spelling pubmed-34581962013-07-15 Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice Aguor, Eissa N. E. Arslan, Fatih van de Kolk, Cees W. A. Nederhoff, Marcel G. J. Doevendans, Pieter A. van Echteld, Cees J. A. Pasterkamp, Gerard Strijkers, Gustav J. MAGMA Research Article OBJECT: Imaging of myocardial infarct composition is essential to assess efficacy of emerging therapeutics. T (2)(*) mapping has the potential to image myocardial hemorrhage and fibrosis by virtue of its short T (2)(*). We aimed to quantify T (2)(*) in acute and chronic myocardial ischemia/reperfusion (I/R) injury in mice. MATERIALS AND METHODS: I/R-injury was induced in C57BL/6 mice (n = 9). Sham-operated mice (n = 8) served as controls. MRI was performed at baseline, and 1, 7 and 28 days after surgery. MRI at 9.4 T consisted of Cine, T (2)(*) mapping and late-gadolinium-enhancement (LGE). Mice (n = 6) were histologically assessed for hemorrhage and collagen in the fibrotic scar. RESULTS: Baseline T (2)(*) values were 17.1 ± 2.0 ms. At day 1, LGE displayed a homogeneous infarct enhancement. T (2)(*) in infarct (12.0 ± 1.1 ms) and remote myocardium (13.9 ± 0.8 ms) was lower than at baseline. On days 7 and 28, LGE was heterogeneous. T (2)(*) in the infarct decreased to 7.9 ± 0.7 and 6.4 ± 0.7 ms, whereas T (2)(*) values in the remote myocardium were 14.2 ± 1.1 and 15.6 ± 1.0 ms. Histology revealed deposition of iron and collagen in parallel with decreased T (2)(*). CONCLUSION: T (2)(*) values are dynamic during infarct development and decrease significantly during scar maturation. In the acute phase, T (2)(*) values in infarcted myocardium differ significantly from those in the chronic phase. T (2)(*) mapping was able to confirm the presence of a chronic infarction in cases where LGE was inconclusive. Hence, T (2)(*) may be used to discriminate between acute and chronic infarctions. Springer-Verlag 2012-02-11 2012 /pmc/articles/PMC3458196/ /pubmed/22327962 http://dx.doi.org/10.1007/s10334-012-0304-0 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Research Article
Aguor, Eissa N. E.
Arslan, Fatih
van de Kolk, Cees W. A.
Nederhoff, Marcel G. J.
Doevendans, Pieter A.
van Echteld, Cees J. A.
Pasterkamp, Gerard
Strijkers, Gustav J.
Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title_full Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title_fullStr Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title_full_unstemmed Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title_short Quantitative T(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
title_sort quantitative t(2)(*) assessment of acute and chronic myocardial ischemia/reperfusion injury in mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458196/
https://www.ncbi.nlm.nih.gov/pubmed/22327962
http://dx.doi.org/10.1007/s10334-012-0304-0
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