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Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer

BACKGROUND: The metastasis-promoting protein S100A4 induces expression of ephrin-A1 and osteopontin in osteosarcoma cell lines. The aim of this study was to investigate S100A4-mediated stimulation of ephrin-A1 and osteopontin in non-small cell lung cancer (NSCLC) cell lines, and to characterize the...

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Autores principales: Rud, Ane Kongsgaard, Lund-Iversen, Marius, Berge, Gisle, Brustugun, Odd Terje, Solberg, Steinar K, Mælandsmo, Gunhild M, Boye, Kjetil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458900/
https://www.ncbi.nlm.nih.gov/pubmed/22853000
http://dx.doi.org/10.1186/1471-2407-12-333
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author Rud, Ane Kongsgaard
Lund-Iversen, Marius
Berge, Gisle
Brustugun, Odd Terje
Solberg, Steinar K
Mælandsmo, Gunhild M
Boye, Kjetil
author_facet Rud, Ane Kongsgaard
Lund-Iversen, Marius
Berge, Gisle
Brustugun, Odd Terje
Solberg, Steinar K
Mælandsmo, Gunhild M
Boye, Kjetil
author_sort Rud, Ane Kongsgaard
collection PubMed
description BACKGROUND: The metastasis-promoting protein S100A4 induces expression of ephrin-A1 and osteopontin in osteosarcoma cell lines. The aim of this study was to investigate S100A4-mediated stimulation of ephrin-A1 and osteopontin in non-small cell lung cancer (NSCLC) cell lines, and to characterize the expression of these biomarkers in primary tumor tissue from NSCLC patients. METHODS: Four NSCLC cell lines were treated with extracellular S100A4, and ephrin-A1 and osteopontin expression was analyzed by real time RT-PCR and Western blotting. Immunohistochemical staining for S100A4, ephrin-A1 and osteopontin was performed on tissue microarrays containing primary tumor samples from a cohort of 217 prospectively recruited NSCLC patients, and associations with clinicopathological parameters were investigated. RESULTS: S100A4 induced ephrin-A1 mRNA and protein expression in adenocarcinoma, but not in squamous carcinoma cell lines, whereas the level of osteopontin was unaffected by S100A4 treatment. In primary tumors, moderate or strong immunoreactivity was observed in 57% of cases for cytoplasmic S100A4, 46% for nuclear S100A4, 86% for ephrin-A1 and 77% for osteopontin. Interestingly, S100A4 expression was associated with ephrin-A1 also in vivo, but there was no association between S100A4 and osteopontin. Expression levels of S100A4 and ephrin-A1 were significantly higher in adenocarcinomas compared to other histological subtypes, and S100A4-positive tumors were smaller and more differentiated than tumors without expression. CONCLUSIONS: Our findings suggest that S100A4, ephrin-A1 and osteopontin are involved in the biology of NSCLC, and further investigation of their potential use as biomarkers in NSCLC is warranted.
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spelling pubmed-34589002012-09-27 Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer Rud, Ane Kongsgaard Lund-Iversen, Marius Berge, Gisle Brustugun, Odd Terje Solberg, Steinar K Mælandsmo, Gunhild M Boye, Kjetil BMC Cancer Research Article BACKGROUND: The metastasis-promoting protein S100A4 induces expression of ephrin-A1 and osteopontin in osteosarcoma cell lines. The aim of this study was to investigate S100A4-mediated stimulation of ephrin-A1 and osteopontin in non-small cell lung cancer (NSCLC) cell lines, and to characterize the expression of these biomarkers in primary tumor tissue from NSCLC patients. METHODS: Four NSCLC cell lines were treated with extracellular S100A4, and ephrin-A1 and osteopontin expression was analyzed by real time RT-PCR and Western blotting. Immunohistochemical staining for S100A4, ephrin-A1 and osteopontin was performed on tissue microarrays containing primary tumor samples from a cohort of 217 prospectively recruited NSCLC patients, and associations with clinicopathological parameters were investigated. RESULTS: S100A4 induced ephrin-A1 mRNA and protein expression in adenocarcinoma, but not in squamous carcinoma cell lines, whereas the level of osteopontin was unaffected by S100A4 treatment. In primary tumors, moderate or strong immunoreactivity was observed in 57% of cases for cytoplasmic S100A4, 46% for nuclear S100A4, 86% for ephrin-A1 and 77% for osteopontin. Interestingly, S100A4 expression was associated with ephrin-A1 also in vivo, but there was no association between S100A4 and osteopontin. Expression levels of S100A4 and ephrin-A1 were significantly higher in adenocarcinomas compared to other histological subtypes, and S100A4-positive tumors were smaller and more differentiated than tumors without expression. CONCLUSIONS: Our findings suggest that S100A4, ephrin-A1 and osteopontin are involved in the biology of NSCLC, and further investigation of their potential use as biomarkers in NSCLC is warranted. BioMed Central 2012-08-01 /pmc/articles/PMC3458900/ /pubmed/22853000 http://dx.doi.org/10.1186/1471-2407-12-333 Text en Copyright ©2012 Rud et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rud, Ane Kongsgaard
Lund-Iversen, Marius
Berge, Gisle
Brustugun, Odd Terje
Solberg, Steinar K
Mælandsmo, Gunhild M
Boye, Kjetil
Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title_full Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title_fullStr Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title_full_unstemmed Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title_short Expression of S100A4, ephrin-A1 and osteopontin in non-small cell lung cancer
title_sort expression of s100a4, ephrin-a1 and osteopontin in non-small cell lung cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458900/
https://www.ncbi.nlm.nih.gov/pubmed/22853000
http://dx.doi.org/10.1186/1471-2407-12-333
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