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Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum
Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%–80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “m...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459414/ https://www.ncbi.nlm.nih.gov/pubmed/23055803 http://dx.doi.org/10.1590/S1415-47572012005000038 |
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author | Kajihara, Daniela de Godoy, Fabiana Hamaji, Thais Alves Blanco, Silvia Regina Van Sluys, Marie-Anne Rossi, Magdalena |
author_facet | Kajihara, Daniela de Godoy, Fabiana Hamaji, Thais Alves Blanco, Silvia Regina Van Sluys, Marie-Anne Rossi, Magdalena |
author_sort | Kajihara, Daniela |
collection | PubMed |
description | Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%–80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of this gene family. This analysis identified orthologous groups and showed that mustang genes are highly conserved in grass genomes. We also explored the transcriptional activity of sugarcane mustang genes in heterologous and homologous systems. These genes were found to be ubiquitously transcribed, with shoot apical meristem having the highest expression levels, and were downregulated by phytohormones. Together, these findings suggest the possible involvement of mustang proteins in the maintenance of hormonal homeostasis. |
format | Online Article Text |
id | pubmed-3459414 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-34594142012-10-10 Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum Kajihara, Daniela de Godoy, Fabiana Hamaji, Thais Alves Blanco, Silvia Regina Van Sluys, Marie-Anne Rossi, Magdalena Genet Mol Biol Plant Genetics Transposable elements (TEs) account for a large portion of plant genomes, particularly in grasses, in which they correspond to 50%–80% of the genomic content. TEs have recently been shown to be a source of new genes and new regulatory networks. The most striking contribution of TEs is referred as “molecular domestication”, by which the element coding sequence loses its movement capacity and acquires cellular function. Recently, domesticated transposases known as mustang and derived from the Mutator element have been described in sugarcane. In order to improve our understanding of the function of these proteins, we identified mustang genes from Sorghum bicolor and Zea mays and performed a phenetic analysis to assess the diversity and evolutionary history of this gene family. This analysis identified orthologous groups and showed that mustang genes are highly conserved in grass genomes. We also explored the transcriptional activity of sugarcane mustang genes in heterologous and homologous systems. These genes were found to be ubiquitously transcribed, with shoot apical meristem having the highest expression levels, and were downregulated by phytohormones. Together, these findings suggest the possible involvement of mustang proteins in the maintenance of hormonal homeostasis. Sociedade Brasileira de Genética 2012 2012-07-05 /pmc/articles/PMC3459414/ /pubmed/23055803 http://dx.doi.org/10.1590/S1415-47572012005000038 Text en Copyright © 2012, Sociedade Brasileira de Genética. License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Plant Genetics Kajihara, Daniela de Godoy, Fabiana Hamaji, Thais Alves Blanco, Silvia Regina Van Sluys, Marie-Anne Rossi, Magdalena Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title | Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title_full | Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title_fullStr | Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title_full_unstemmed | Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title_short | Functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
title_sort | functional characterization of sugarcane mustang domesticated transposases and comparative diversity in sugarcane, rice, maize and sorghum |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459414/ https://www.ncbi.nlm.nih.gov/pubmed/23055803 http://dx.doi.org/10.1590/S1415-47572012005000038 |
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