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Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease

OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five s...

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Autores principales: Ricanek, Petr, Lothe, Sheba M, Frye, Stephan A, Rydning, Andreas, Vatn, Morten H, Tønjum, Tone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459595/
https://www.ncbi.nlm.nih.gov/pubmed/23049264
http://dx.doi.org/10.2147/CEG.S33858
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author Ricanek, Petr
Lothe, Sheba M
Frye, Stephan A
Rydning, Andreas
Vatn, Morten H
Tønjum, Tone
author_facet Ricanek, Petr
Lothe, Sheba M
Frye, Stephan A
Rydning, Andreas
Vatn, Morten H
Tønjum, Tone
author_sort Ricanek, Petr
collection PubMed
description OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed. RESULTS: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control). CONCLUSION: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries.
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spelling pubmed-34595952012-10-05 Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease Ricanek, Petr Lothe, Sheba M Frye, Stephan A Rydning, Andreas Vatn, Morten H Tønjum, Tone Clin Exp Gastroenterol Original Research OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed. RESULTS: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control). CONCLUSION: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries. Dove Medical Press 2012-09-24 /pmc/articles/PMC3459595/ /pubmed/23049264 http://dx.doi.org/10.2147/CEG.S33858 Text en © 2012 Ricanek et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Ricanek, Petr
Lothe, Sheba M
Frye, Stephan A
Rydning, Andreas
Vatn, Morten H
Tønjum, Tone
Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title_full Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title_fullStr Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title_full_unstemmed Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title_short Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
title_sort gut bacterial profile in patients newly diagnosed with treatment-naïve crohn’s disease
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459595/
https://www.ncbi.nlm.nih.gov/pubmed/23049264
http://dx.doi.org/10.2147/CEG.S33858
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