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Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease
OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459595/ https://www.ncbi.nlm.nih.gov/pubmed/23049264 http://dx.doi.org/10.2147/CEG.S33858 |
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author | Ricanek, Petr Lothe, Sheba M Frye, Stephan A Rydning, Andreas Vatn, Morten H Tønjum, Tone |
author_facet | Ricanek, Petr Lothe, Sheba M Frye, Stephan A Rydning, Andreas Vatn, Morten H Tønjum, Tone |
author_sort | Ricanek, Petr |
collection | PubMed |
description | OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed. RESULTS: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control). CONCLUSION: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries. |
format | Online Article Text |
id | pubmed-3459595 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34595952012-10-05 Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease Ricanek, Petr Lothe, Sheba M Frye, Stephan A Rydning, Andreas Vatn, Morten H Tønjum, Tone Clin Exp Gastroenterol Original Research OBJECTIVES: The aim of this study was to define the composition of the gut bacterial flora in Norwegian patients with early stage Crohn’s disease (CD). METHODS: By using a nonselective metagenomics approach, the general bacterial composition in mucosal biopsies from the ileum and the colon of five subjects, four patients with different phenotypes of CD, and one noninflammatory bowel disease control, was characterized. After partial 16S ribosomal RNA (rRNA) gene sequencing, BLAST homology searches for species identification and phylogenetic analysis were performed. RESULTS: An overall biodiversity of 106 different bacterial operational taxonomic units (OTUs) was detected in the cloned libraries. Nearly all OTUs belonged to the phylae Bacteroidetes (42% in CD, 71% in the control) or Firmicutes (42% in CD, 28% in the control), except for some OTUs that belonged to the phylum Proteobacteria (15% in CD, 0% in the control) and a few OTUs that could not be assigned to a phylum (2% in CD, 1% in the control). CONCLUSION: Based on the high incidence of inflammatory bowel disease (IBD) in Norway, this pilot study represents a relevant determination of the gut microbiota in Norwegian patients compared to previous findings in other countries. The bacterial profile of Norwegian CD patients was found to be similar to that of CD patients in other countries. The findings do not support a particular bacterial composition as a predominant causative factor for the high incidence of IBD that exists in some countries. Dove Medical Press 2012-09-24 /pmc/articles/PMC3459595/ /pubmed/23049264 http://dx.doi.org/10.2147/CEG.S33858 Text en © 2012 Ricanek et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited. |
spellingShingle | Original Research Ricanek, Petr Lothe, Sheba M Frye, Stephan A Rydning, Andreas Vatn, Morten H Tønjum, Tone Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title | Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title_full | Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title_fullStr | Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title_full_unstemmed | Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title_short | Gut bacterial profile in patients newly diagnosed with treatment-naïve Crohn’s disease |
title_sort | gut bacterial profile in patients newly diagnosed with treatment-naïve crohn’s disease |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459595/ https://www.ncbi.nlm.nih.gov/pubmed/23049264 http://dx.doi.org/10.2147/CEG.S33858 |
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