Cargando…

No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients

BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provide...

Descripción completa

Detalles Bibliográficos
Autores principales: Nissen, Kari K, Laska, Magdalena J, Hansen, Bettina, Pedersen, Finn S, Nexø, Bjørn A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459695/
https://www.ncbi.nlm.nih.gov/pubmed/22958517
http://dx.doi.org/10.1186/1743-422X-9-188
_version_ 1782244840682029056
author Nissen, Kari K
Laska, Magdalena J
Hansen, Bettina
Pedersen, Finn S
Nexø, Bjørn A
author_facet Nissen, Kari K
Laska, Magdalena J
Hansen, Bettina
Pedersen, Finn S
Nexø, Bjørn A
author_sort Nissen, Kari K
collection PubMed
description BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provided genetic evidence for association between the endogenous retrovirus HERV-Fc1 and MS, suggesting that HERV-Fc1 plays a role in this multifactorial disease. We have found increased expression of HERV-Fc1 in MS patients suffering from recent attack, but the underlying mechanism for association is still unknown. FINDINGS: Evidence from animal models indicates that ERV implication in the pathogenesis of diseases can be a result of extra copies of the virus in the germ line. Therefore, we investigated the genome of 81 individuals, 74 patients with MS and 7 healthy controls, by means of Southern blotting, for presence of extra HERV-Fc1 copies. The known insertion at the Xq21.33 position was readily detectable, but no additional insertions in other genomic contexts could be identified in any studied individuals. This substantiates our previous copy-number PCR findings of a 2:1 ratio of HERV-Fc1 DNA between women and men, as expected from the X-chromosome location; there was no difference between patient and control individuals. CONCLUSIONS: No additional germ line copies of HERV-Fc1 could be identified, precluding such copies to underlie the association between this provirus and multiples sclerosis.
format Online
Article
Text
id pubmed-3459695
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34596952012-09-28 No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients Nissen, Kari K Laska, Magdalena J Hansen, Bettina Pedersen, Finn S Nexø, Bjørn A Virol J Short Report BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provided genetic evidence for association between the endogenous retrovirus HERV-Fc1 and MS, suggesting that HERV-Fc1 plays a role in this multifactorial disease. We have found increased expression of HERV-Fc1 in MS patients suffering from recent attack, but the underlying mechanism for association is still unknown. FINDINGS: Evidence from animal models indicates that ERV implication in the pathogenesis of diseases can be a result of extra copies of the virus in the germ line. Therefore, we investigated the genome of 81 individuals, 74 patients with MS and 7 healthy controls, by means of Southern blotting, for presence of extra HERV-Fc1 copies. The known insertion at the Xq21.33 position was readily detectable, but no additional insertions in other genomic contexts could be identified in any studied individuals. This substantiates our previous copy-number PCR findings of a 2:1 ratio of HERV-Fc1 DNA between women and men, as expected from the X-chromosome location; there was no difference between patient and control individuals. CONCLUSIONS: No additional germ line copies of HERV-Fc1 could be identified, precluding such copies to underlie the association between this provirus and multiples sclerosis. BioMed Central 2012-09-08 /pmc/articles/PMC3459695/ /pubmed/22958517 http://dx.doi.org/10.1186/1743-422X-9-188 Text en Copyright ©2012 Nissen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Report
Nissen, Kari K
Laska, Magdalena J
Hansen, Bettina
Pedersen, Finn S
Nexø, Bjørn A
No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title_full No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title_fullStr No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title_full_unstemmed No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title_short No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
title_sort no additional copies of herv-fc1 in the germ line of multiple sclerosis patients
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459695/
https://www.ncbi.nlm.nih.gov/pubmed/22958517
http://dx.doi.org/10.1186/1743-422X-9-188
work_keys_str_mv AT nissenkarik noadditionalcopiesofhervfc1inthegermlineofmultiplesclerosispatients
AT laskamagdalenaj noadditionalcopiesofhervfc1inthegermlineofmultiplesclerosispatients
AT hansenbettina noadditionalcopiesofhervfc1inthegermlineofmultiplesclerosispatients
AT pedersenfinns noadditionalcopiesofhervfc1inthegermlineofmultiplesclerosispatients
AT nexøbjørna noadditionalcopiesofhervfc1inthegermlineofmultiplesclerosispatients