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No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients
BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provide...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459695/ https://www.ncbi.nlm.nih.gov/pubmed/22958517 http://dx.doi.org/10.1186/1743-422X-9-188 |
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author | Nissen, Kari K Laska, Magdalena J Hansen, Bettina Pedersen, Finn S Nexø, Bjørn A |
author_facet | Nissen, Kari K Laska, Magdalena J Hansen, Bettina Pedersen, Finn S Nexø, Bjørn A |
author_sort | Nissen, Kari K |
collection | PubMed |
description | BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provided genetic evidence for association between the endogenous retrovirus HERV-Fc1 and MS, suggesting that HERV-Fc1 plays a role in this multifactorial disease. We have found increased expression of HERV-Fc1 in MS patients suffering from recent attack, but the underlying mechanism for association is still unknown. FINDINGS: Evidence from animal models indicates that ERV implication in the pathogenesis of diseases can be a result of extra copies of the virus in the germ line. Therefore, we investigated the genome of 81 individuals, 74 patients with MS and 7 healthy controls, by means of Southern blotting, for presence of extra HERV-Fc1 copies. The known insertion at the Xq21.33 position was readily detectable, but no additional insertions in other genomic contexts could be identified in any studied individuals. This substantiates our previous copy-number PCR findings of a 2:1 ratio of HERV-Fc1 DNA between women and men, as expected from the X-chromosome location; there was no difference between patient and control individuals. CONCLUSIONS: No additional germ line copies of HERV-Fc1 could be identified, precluding such copies to underlie the association between this provirus and multiples sclerosis. |
format | Online Article Text |
id | pubmed-3459695 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34596952012-09-28 No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients Nissen, Kari K Laska, Magdalena J Hansen, Bettina Pedersen, Finn S Nexø, Bjørn A Virol J Short Report BACKGROUND: Human endogenous retroviruses (HERVs) are suspected to play a role in the development of multiple sclerosis (MS). This suspicion has in part been based on increased expression of viral RNA or proteins or antibodies targeting retroviral products in MS patients. Recently, our group provided genetic evidence for association between the endogenous retrovirus HERV-Fc1 and MS, suggesting that HERV-Fc1 plays a role in this multifactorial disease. We have found increased expression of HERV-Fc1 in MS patients suffering from recent attack, but the underlying mechanism for association is still unknown. FINDINGS: Evidence from animal models indicates that ERV implication in the pathogenesis of diseases can be a result of extra copies of the virus in the germ line. Therefore, we investigated the genome of 81 individuals, 74 patients with MS and 7 healthy controls, by means of Southern blotting, for presence of extra HERV-Fc1 copies. The known insertion at the Xq21.33 position was readily detectable, but no additional insertions in other genomic contexts could be identified in any studied individuals. This substantiates our previous copy-number PCR findings of a 2:1 ratio of HERV-Fc1 DNA between women and men, as expected from the X-chromosome location; there was no difference between patient and control individuals. CONCLUSIONS: No additional germ line copies of HERV-Fc1 could be identified, precluding such copies to underlie the association between this provirus and multiples sclerosis. BioMed Central 2012-09-08 /pmc/articles/PMC3459695/ /pubmed/22958517 http://dx.doi.org/10.1186/1743-422X-9-188 Text en Copyright ©2012 Nissen et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Nissen, Kari K Laska, Magdalena J Hansen, Bettina Pedersen, Finn S Nexø, Bjørn A No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title | No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title_full | No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title_fullStr | No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title_full_unstemmed | No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title_short | No additional copies of HERV-Fc1 in the germ line of multiple sclerosis patients |
title_sort | no additional copies of herv-fc1 in the germ line of multiple sclerosis patients |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459695/ https://www.ncbi.nlm.nih.gov/pubmed/22958517 http://dx.doi.org/10.1186/1743-422X-9-188 |
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