FRUIT, a Scar-Free System for Targeted Chromosomal Mutagenesis, Epitope Tagging, and Promoter Replacement in Escherichia coli and Salmonella enterica

Recombineering is a widely-used approach to delete genes, introduce insertions and point mutations, and introduce epitope tags into bacterial chromosomes. Many recombineering methods have been described, for a wide range of bacterial species. These methods are often limited by (i) low efficiency, an...

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Detalles Bibliográficos
Autores principales: Stringer, Anne M., Singh, Navjot, Yermakova, Anastasiya, Petrone, Brianna L., Amarasinghe, Jayaleka J., Reyes-Diaz, Lucia, Mantis, Nicholas J., Wade, Joseph T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459970/
https://www.ncbi.nlm.nih.gov/pubmed/23028641
http://dx.doi.org/10.1371/journal.pone.0044841

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