A Mimicking-of-DNA-Methylation-Patterns Pipeline for Overcoming the Restriction Barrier of Bacteria

Genetic transformation of bacteria harboring multiple Restriction-Modification (R-M) systems is often difficult using conventional methods. Here, we describe a mimicking-of-DNA-methylation-patterns (MoDMP) pipeline to address this problem in three difficult-to-transform bacterial strains. Twenty-fou...

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Detalles Bibliográficos
Autores principales: Zhang, Guoqiang, Wang, Wenzhao, Deng, Aihua, Sun, Zhaopeng, Zhang, Yun, Liang, Yong, Che, Yongsheng, Wen, Tingyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3459991/
https://www.ncbi.nlm.nih.gov/pubmed/23028379
http://dx.doi.org/10.1371/journal.pgen.1002987

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