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Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline
BACKGROUND: Sickle cell disease is a hemoglobinopathy characterized by hemolytic anemia, increased susceptibility to infections and recurrent vaso-occlusive crises that reduces the quality of life of sufferers. OBJECTIVE: To evaluate the correlation of the levels of lactate dehydrogenase, malonaldeh...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Hematologia e Hemoterapia
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460400/ https://www.ncbi.nlm.nih.gov/pubmed/23049438 http://dx.doi.org/10.5581/1516-8484.20120069 |
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author | Elias, Darcielle Bruna Dias Rocha, Lilianne Brito da Silva Cavalcante, Maritza Barbosa Pedrosa, Alano Martins Justino, Izabel Cristina Bandeira Gonçalves, Romélia Pinheiro |
author_facet | Elias, Darcielle Bruna Dias Rocha, Lilianne Brito da Silva Cavalcante, Maritza Barbosa Pedrosa, Alano Martins Justino, Izabel Cristina Bandeira Gonçalves, Romélia Pinheiro |
author_sort | Elias, Darcielle Bruna Dias |
collection | PubMed |
description | BACKGROUND: Sickle cell disease is a hemoglobinopathy characterized by hemolytic anemia, increased susceptibility to infections and recurrent vaso-occlusive crises that reduces the quality of life of sufferers. OBJECTIVE: To evaluate the correlation of the levels of lactate dehydrogenase, malonaldehyde and nitrite to fetal hemoglobin in patients with sickle cell disease not under treatment with hydroxyurea in outpatients at a university hospital in Fortaleza, Ceará, Brazil. METHODS: Forty-four patients diagnosed with sickle cell disease were enrolled at baseline. Diagnosis was confirmed by evaluating the beta globin gene using polymerase chain reaction-restriction fragment length polymorphism. The concentration of fetal hemoglobin was obtained by high-performance liquid chromatography. Serum levels of nitrite, malonaldehyde and lactate dehydrogenase were measured by biochemical methods. RESULTS: Significantly higher levels of lactate dehydrogenase, nitrite and malonaldehyde were observed in patients with sickle cell disease compared to a control group. The study of the correlation between fetal hemoglobin levels and these variables showed a negative correlation with nitrite levels. No correlation was found between fetal hemoglobin and malonaldehyde or lactate dehydrogenase. When the study population was stratified according to fetal hemoglobin levels, a decrease in the levels of nitrite was observed with higher levels of fetal hemoglobin (p-value = 0.0415). CONCLUSION: The results show that, similar to fetal hemoglobin levels, the concentration of nitrite can predict the clinical course of the disease, but should not be used alone as a modulator of prognosis in patients with sickle cell disease. |
format | Online Article Text |
id | pubmed-3460400 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Associação Brasileira de Hematologia e Hemoterapia |
record_format | MEDLINE/PubMed |
spelling | pubmed-34604002012-10-04 Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline Elias, Darcielle Bruna Dias Rocha, Lilianne Brito da Silva Cavalcante, Maritza Barbosa Pedrosa, Alano Martins Justino, Izabel Cristina Bandeira Gonçalves, Romélia Pinheiro Rev Bras Hematol Hemoter Original Article BACKGROUND: Sickle cell disease is a hemoglobinopathy characterized by hemolytic anemia, increased susceptibility to infections and recurrent vaso-occlusive crises that reduces the quality of life of sufferers. OBJECTIVE: To evaluate the correlation of the levels of lactate dehydrogenase, malonaldehyde and nitrite to fetal hemoglobin in patients with sickle cell disease not under treatment with hydroxyurea in outpatients at a university hospital in Fortaleza, Ceará, Brazil. METHODS: Forty-four patients diagnosed with sickle cell disease were enrolled at baseline. Diagnosis was confirmed by evaluating the beta globin gene using polymerase chain reaction-restriction fragment length polymorphism. The concentration of fetal hemoglobin was obtained by high-performance liquid chromatography. Serum levels of nitrite, malonaldehyde and lactate dehydrogenase were measured by biochemical methods. RESULTS: Significantly higher levels of lactate dehydrogenase, nitrite and malonaldehyde were observed in patients with sickle cell disease compared to a control group. The study of the correlation between fetal hemoglobin levels and these variables showed a negative correlation with nitrite levels. No correlation was found between fetal hemoglobin and malonaldehyde or lactate dehydrogenase. When the study population was stratified according to fetal hemoglobin levels, a decrease in the levels of nitrite was observed with higher levels of fetal hemoglobin (p-value = 0.0415). CONCLUSION: The results show that, similar to fetal hemoglobin levels, the concentration of nitrite can predict the clinical course of the disease, but should not be used alone as a modulator of prognosis in patients with sickle cell disease. Associação Brasileira de Hematologia e Hemoterapia 2012 /pmc/articles/PMC3460400/ /pubmed/23049438 http://dx.doi.org/10.5581/1516-8484.20120069 Text en http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Elias, Darcielle Bruna Dias Rocha, Lilianne Brito da Silva Cavalcante, Maritza Barbosa Pedrosa, Alano Martins Justino, Izabel Cristina Bandeira Gonçalves, Romélia Pinheiro Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title | Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title_full | Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title_fullStr | Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title_full_unstemmed | Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title_short | Correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
title_sort | correlation of low levels of nitrite and high levels of fetal hemoglobin in patients with sickle cell disease at baseline |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460400/ https://www.ncbi.nlm.nih.gov/pubmed/23049438 http://dx.doi.org/10.5581/1516-8484.20120069 |
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