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Impact of endothelial lipase on cellular lipid composition

Using mass spectrometry (MS), we examined the impact of endothelial lipase (EL) overexpression on the cellular phospholipid (PL) and triglyceride (TG) content of human aortic endothelial cells (HAEC) and of mouse plasma and liver tissue. In HAEC incubated with the major EL substrate, HDL, adenovirus...

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Autores principales: Riederer, Monika, Köfeler, Harald, Lechleitner, Margarete, Tritscher, Michaela, Frank, Saša
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Pub. Co 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460414/
https://www.ncbi.nlm.nih.gov/pubmed/23075452
http://dx.doi.org/10.1016/j.bbalip.2012.03.006
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author Riederer, Monika
Köfeler, Harald
Lechleitner, Margarete
Tritscher, Michaela
Frank, Saša
author_facet Riederer, Monika
Köfeler, Harald
Lechleitner, Margarete
Tritscher, Michaela
Frank, Saša
author_sort Riederer, Monika
collection PubMed
description Using mass spectrometry (MS), we examined the impact of endothelial lipase (EL) overexpression on the cellular phospholipid (PL) and triglyceride (TG) content of human aortic endothelial cells (HAEC) and of mouse plasma and liver tissue. In HAEC incubated with the major EL substrate, HDL, adenovirus (Ad)-mediated EL overexpression resulted in the generation of various lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) species in cell culture supernatants. While the cellular phosphatidylethanolamine (PE) content remained unaltered, cellular phosphatidylcholine (PC)-, LPC- and TG-contents were significantly increased upon EL overexpression. Importantly, cellular lipid composition was not altered when EL was overexpressed in the absence of HDL. [(14)C]-LPC accumulated in EL overexpressing, but not LacZ-control cells, incubated with [(14)C]-PC labeled HDL, indicating EL-mediated LPC supply. Exogenously added [(14)C]-LPC accumulated in HAEC as well. Its conversion to [(14)C]-PC was sensitive to a lysophospholipid acyltransferase (LPLAT) inhibitor, thimerosal. Incorporation of [(3)H]-Choline into cellular PC was 56% lower in EL compared with LacZ cells, indicating decreased endogenous PC synthesis. In mice, adenovirus mediated EL overexpression decreased plasma PC, PE and LPC and increased liver LPC, LPE and TG content. Based on our results, we conclude that EL not only supplies cells with FFA as found previously, but also with HDL-derived LPC and LPE species resulting in increased cellular TG and PC content as well as decreased endogenous PC synthesis.
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spelling pubmed-34604142012-09-28 Impact of endothelial lipase on cellular lipid composition Riederer, Monika Köfeler, Harald Lechleitner, Margarete Tritscher, Michaela Frank, Saša Biochim Biophys Acta Article Using mass spectrometry (MS), we examined the impact of endothelial lipase (EL) overexpression on the cellular phospholipid (PL) and triglyceride (TG) content of human aortic endothelial cells (HAEC) and of mouse plasma and liver tissue. In HAEC incubated with the major EL substrate, HDL, adenovirus (Ad)-mediated EL overexpression resulted in the generation of various lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE) species in cell culture supernatants. While the cellular phosphatidylethanolamine (PE) content remained unaltered, cellular phosphatidylcholine (PC)-, LPC- and TG-contents were significantly increased upon EL overexpression. Importantly, cellular lipid composition was not altered when EL was overexpressed in the absence of HDL. [(14)C]-LPC accumulated in EL overexpressing, but not LacZ-control cells, incubated with [(14)C]-PC labeled HDL, indicating EL-mediated LPC supply. Exogenously added [(14)C]-LPC accumulated in HAEC as well. Its conversion to [(14)C]-PC was sensitive to a lysophospholipid acyltransferase (LPLAT) inhibitor, thimerosal. Incorporation of [(3)H]-Choline into cellular PC was 56% lower in EL compared with LacZ cells, indicating decreased endogenous PC synthesis. In mice, adenovirus mediated EL overexpression decreased plasma PC, PE and LPC and increased liver LPC, LPE and TG content. Based on our results, we conclude that EL not only supplies cells with FFA as found previously, but also with HDL-derived LPC and LPE species resulting in increased cellular TG and PC content as well as decreased endogenous PC synthesis. Elsevier Pub. Co 2012-07 /pmc/articles/PMC3460414/ /pubmed/23075452 http://dx.doi.org/10.1016/j.bbalip.2012.03.006 Text en © 2012 Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Riederer, Monika
Köfeler, Harald
Lechleitner, Margarete
Tritscher, Michaela
Frank, Saša
Impact of endothelial lipase on cellular lipid composition
title Impact of endothelial lipase on cellular lipid composition
title_full Impact of endothelial lipase on cellular lipid composition
title_fullStr Impact of endothelial lipase on cellular lipid composition
title_full_unstemmed Impact of endothelial lipase on cellular lipid composition
title_short Impact of endothelial lipase on cellular lipid composition
title_sort impact of endothelial lipase on cellular lipid composition
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460414/
https://www.ncbi.nlm.nih.gov/pubmed/23075452
http://dx.doi.org/10.1016/j.bbalip.2012.03.006
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