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Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the succ...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460531/ https://www.ncbi.nlm.nih.gov/pubmed/23024930 http://dx.doi.org/10.1007/s12668-011-0010-9 |
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author | Lai, Wing-Fu Tang, Gu-Ping Wang, Xin Li, Guo Yao, Hong Shen, Zan Lu, Gang Poon, Wai Sang Kung, Hsiang-Fu Lin, Marie C. M. |
author_facet | Lai, Wing-Fu Tang, Gu-Ping Wang, Xin Li, Guo Yao, Hong Shen, Zan Lu, Gang Poon, Wai Sang Kung, Hsiang-Fu Lin, Marie C. M. |
author_sort | Lai, Wing-Fu |
collection | PubMed |
description | This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8–3.2 ppm), CyD (at δ 5.2, 3.8–4.0 and 3.4–3.6 ppm) and TAT (at δ 1.6–1.9 and 6.8–7.2 ppm) in the (1)H NMR spectrum of TAT-PEI-β-CyD. The polymer–plasmid–DNA polyplex could condense DNA at an N/P ratio of 7.0–8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12668-011-0010-9) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-3460531 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-34605312012-09-28 Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells Lai, Wing-Fu Tang, Gu-Ping Wang, Xin Li, Guo Yao, Hong Shen, Zan Lu, Gang Poon, Wai Sang Kung, Hsiang-Fu Lin, Marie C. M. Bionanoscience Article This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8–3.2 ppm), CyD (at δ 5.2, 3.8–4.0 and 3.4–3.6 ppm) and TAT (at δ 1.6–1.9 and 6.8–7.2 ppm) in the (1)H NMR spectrum of TAT-PEI-β-CyD. The polymer–plasmid–DNA polyplex could condense DNA at an N/P ratio of 7.0–8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12668-011-0010-9) contains supplementary material, which is available to authorized users. Springer US 2011-06-18 2011 /pmc/articles/PMC3460531/ /pubmed/23024930 http://dx.doi.org/10.1007/s12668-011-0010-9 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Lai, Wing-Fu Tang, Gu-Ping Wang, Xin Li, Guo Yao, Hong Shen, Zan Lu, Gang Poon, Wai Sang Kung, Hsiang-Fu Lin, Marie C. M. Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title | Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title_full | Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title_fullStr | Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title_full_unstemmed | Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title_short | Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells |
title_sort | cyclodextrin-pei-tat polymer as a vector for plasmid dna delivery to placenta mesenchymal stem cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460531/ https://www.ncbi.nlm.nih.gov/pubmed/23024930 http://dx.doi.org/10.1007/s12668-011-0010-9 |
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