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Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells

This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the succ...

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Autores principales: Lai, Wing-Fu, Tang, Gu-Ping, Wang, Xin, Li, Guo, Yao, Hong, Shen, Zan, Lu, Gang, Poon, Wai Sang, Kung, Hsiang-Fu, Lin, Marie C. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460531/
https://www.ncbi.nlm.nih.gov/pubmed/23024930
http://dx.doi.org/10.1007/s12668-011-0010-9
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author Lai, Wing-Fu
Tang, Gu-Ping
Wang, Xin
Li, Guo
Yao, Hong
Shen, Zan
Lu, Gang
Poon, Wai Sang
Kung, Hsiang-Fu
Lin, Marie C. M.
author_facet Lai, Wing-Fu
Tang, Gu-Ping
Wang, Xin
Li, Guo
Yao, Hong
Shen, Zan
Lu, Gang
Poon, Wai Sang
Kung, Hsiang-Fu
Lin, Marie C. M.
author_sort Lai, Wing-Fu
collection PubMed
description This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8–3.2 ppm), CyD (at δ 5.2, 3.8–4.0 and 3.4–3.6 ppm) and TAT (at δ 1.6–1.9 and 6.8–7.2 ppm) in the (1)H NMR spectrum of TAT-PEI-β-CyD. The polymer–plasmid–DNA polyplex could condense DNA at an N/P ratio of 7.0–8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12668-011-0010-9) contains supplementary material, which is available to authorized users.
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spelling pubmed-34605312012-09-28 Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells Lai, Wing-Fu Tang, Gu-Ping Wang, Xin Li, Guo Yao, Hong Shen, Zan Lu, Gang Poon, Wai Sang Kung, Hsiang-Fu Lin, Marie C. M. Bionanoscience Article This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8–3.2 ppm), CyD (at δ 5.2, 3.8–4.0 and 3.4–3.6 ppm) and TAT (at δ 1.6–1.9 and 6.8–7.2 ppm) in the (1)H NMR spectrum of TAT-PEI-β-CyD. The polymer–plasmid–DNA polyplex could condense DNA at an N/P ratio of 7.0–8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-β-CyD, conjugation of the TAT peptide onto PEI-β-CyD was demonstrated to improve the transfection efficiency of PEI-β-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-β-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-β-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12668-011-0010-9) contains supplementary material, which is available to authorized users. Springer US 2011-06-18 2011 /pmc/articles/PMC3460531/ /pubmed/23024930 http://dx.doi.org/10.1007/s12668-011-0010-9 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Lai, Wing-Fu
Tang, Gu-Ping
Wang, Xin
Li, Guo
Yao, Hong
Shen, Zan
Lu, Gang
Poon, Wai Sang
Kung, Hsiang-Fu
Lin, Marie C. M.
Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title_full Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title_fullStr Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title_full_unstemmed Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title_short Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells
title_sort cyclodextrin-pei-tat polymer as a vector for plasmid dna delivery to placenta mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460531/
https://www.ncbi.nlm.nih.gov/pubmed/23024930
http://dx.doi.org/10.1007/s12668-011-0010-9
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