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Expression and Purification of the Cystic Fibrosis Transmembrane Conductance Regulator Protein in Saccharomyces cerevisiae

The cystic fibrosis transmembrane conductance regulator (CFTR) is a chloride channel, that when mutated, can give rise to cystic fibrosis in humans.There is therefore considerable interest in this protein, but efforts to study its structure and activity have been hampered by the difficulty of expres...

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Detalles Bibliográficos
Autores principales:	O'Ryan, Liam, Rimington, Tracy, Cant, Natasha, Ford, Robert C.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MyJove Corporation 2012
Materias:	Molecular Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460588/ https://www.ncbi.nlm.nih.gov/pubmed/22433465 http://dx.doi.org/10.3791/3860

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