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Partial and Full PCR-Based Reverse Genetics Strategy for Influenza Viruses

Since 1999, plasmid-based reverse genetics (RG) systems have revolutionized the way influenza viruses are studied. However, it is not unusual to encounter cloning difficulties for one or more influenza genes while attempting to recover virus de novo. To overcome some of these shortcomings we sought...

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Detalles Bibliográficos
Autores principales:	Chen, Hongjun, Ye, Jianqiang, Xu, Kemin, Angel, Matthew, Shao, Hongxia, Ferrero, Andrea, Sutton, Troy, Perez, Daniel R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460856/ https://www.ncbi.nlm.nih.gov/pubmed/23029501 http://dx.doi.org/10.1371/journal.pone.0046378

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