Cargando…
Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation
Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs under dynamic hydraulic compression to evaluate and compare osteogenic abilities....
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460891/ https://www.ncbi.nlm.nih.gov/pubmed/23029565 http://dx.doi.org/10.1371/journal.pone.0046689 |
_version_ | 1782245006779613184 |
---|---|
author | Park, Sang-Hyug Sim, Woo Young Min, Byoung-Hyun Yang, Sang Sik Khademhosseini, Ali Kaplan, David L. |
author_facet | Park, Sang-Hyug Sim, Woo Young Min, Byoung-Hyun Yang, Sang Sik Khademhosseini, Ali Kaplan, David L. |
author_sort | Park, Sang-Hyug |
collection | PubMed |
description | Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs under dynamic hydraulic compression to evaluate and compare osteogenic abilities. A novel micro cell chip integrated with microvalves and microscale cell culture chambers separated from an air-pressure chamber was developed using microfabrication technology. The microscale chip enables the culture of two types of stem cells concurrently, where each is loaded into cell culture chambers and dynamic compressive stimulation is applied to the cells uniformly. Dynamic hydraulic compression (1 Hz, 1 psi) increased the production of osteogenic matrix components (bone sialoprotein, oateopontin, type I collagen) and integrin (CD11b and CD31) expression from both stem cell sources. Alkaline phosphatase and Alrizarin red staining were evident in the stimulated hMSCs, while the stimulated hASCs did not show significant increases in staining under the same stimulation conditions. Upon application of mechanical stimulus to the two types of stem cells, integrin (β1) and osteogenic gene markers were upregulated from both cell types. In conclusion, stimulated hMSCs and hASCs showed increased osteogenic gene expression compared to non-stimulated groups. The hMSCs were more sensitive to mechanical stimulation and more effective towards osteogenic differentiation than the hASCs under these modes of mechanical stimulation. |
format | Online Article Text |
id | pubmed-3460891 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-34608912012-10-01 Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation Park, Sang-Hyug Sim, Woo Young Min, Byoung-Hyun Yang, Sang Sik Khademhosseini, Ali Kaplan, David L. PLoS One Research Article Adipose tissue-derived stem cells (ASCs) are considered as an attractive stem cell source for tissue engineering and regenerative medicine. We compared human bone marrow-derived mesenchymal stem cells (hMSCs) and hASCs under dynamic hydraulic compression to evaluate and compare osteogenic abilities. A novel micro cell chip integrated with microvalves and microscale cell culture chambers separated from an air-pressure chamber was developed using microfabrication technology. The microscale chip enables the culture of two types of stem cells concurrently, where each is loaded into cell culture chambers and dynamic compressive stimulation is applied to the cells uniformly. Dynamic hydraulic compression (1 Hz, 1 psi) increased the production of osteogenic matrix components (bone sialoprotein, oateopontin, type I collagen) and integrin (CD11b and CD31) expression from both stem cell sources. Alkaline phosphatase and Alrizarin red staining were evident in the stimulated hMSCs, while the stimulated hASCs did not show significant increases in staining under the same stimulation conditions. Upon application of mechanical stimulus to the two types of stem cells, integrin (β1) and osteogenic gene markers were upregulated from both cell types. In conclusion, stimulated hMSCs and hASCs showed increased osteogenic gene expression compared to non-stimulated groups. The hMSCs were more sensitive to mechanical stimulation and more effective towards osteogenic differentiation than the hASCs under these modes of mechanical stimulation. Public Library of Science 2012-09-28 /pmc/articles/PMC3460891/ /pubmed/23029565 http://dx.doi.org/10.1371/journal.pone.0046689 Text en © 2012 Park et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Park, Sang-Hyug Sim, Woo Young Min, Byoung-Hyun Yang, Sang Sik Khademhosseini, Ali Kaplan, David L. Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title | Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title_full | Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title_fullStr | Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title_full_unstemmed | Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title_short | Chip-Based Comparison of the Osteogenesis of Human Bone Marrow- and Adipose Tissue-Derived Mesenchymal Stem Cells under Mechanical Stimulation |
title_sort | chip-based comparison of the osteogenesis of human bone marrow- and adipose tissue-derived mesenchymal stem cells under mechanical stimulation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460891/ https://www.ncbi.nlm.nih.gov/pubmed/23029565 http://dx.doi.org/10.1371/journal.pone.0046689 |
work_keys_str_mv | AT parksanghyug chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation AT simwooyoung chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation AT minbyounghyun chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation AT yangsangsik chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation AT khademhosseiniali chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation AT kaplandavidl chipbasedcomparisonoftheosteogenesisofhumanbonemarrowandadiposetissuederivedmesenchymalstemcellsundermechanicalstimulation |