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Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates

Polymorphic amplified typing sequences (PATS), a PCR-based Escherichia coli O157:H7 (O157) strain typing system, targets insertions-deletions and single nucleotide polymorphisms at XbaI and AvrII restriction enzyme sites, respectively, and the virulence genes (stx1, stx2, eae, hlyA) in the O157 geno...

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Detalles Bibliográficos
Autores principales:	Kudva, Indira T., Davis, Margaret A., Griffin, Robert W., Garren, Jeonifer, Murray, Megan, John, Manohar, Hovde, Carolyn J., Calderwood, Stephen B.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Hindawi Publishing Corporation 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461292/ https://www.ncbi.nlm.nih.gov/pubmed/23049559 http://dx.doi.org/10.1155/2012/140105

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