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Characterisation of a natural variant of the γ-butyrolactone signalling receptor

BACKGROUND: The control of antibiotic production in Streptomyces coelicolor A3(2) involves complicated regulatory networks with multiple regulators controlling the expression of antibiotic biosynthetic pathways. One such regulatory network is that of the γ-butyrolactones, the so-called S. coelicolor...

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Autores principales: Gottelt, Marco, Hesketh, Andrew, Bunet, Robert, Puri, Pranav, Takano, Eriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461410/
https://www.ncbi.nlm.nih.gov/pubmed/22839725
http://dx.doi.org/10.1186/1756-0500-5-379
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author Gottelt, Marco
Hesketh, Andrew
Bunet, Robert
Puri, Pranav
Takano, Eriko
author_facet Gottelt, Marco
Hesketh, Andrew
Bunet, Robert
Puri, Pranav
Takano, Eriko
author_sort Gottelt, Marco
collection PubMed
description BACKGROUND: The control of antibiotic production in Streptomyces coelicolor A3(2) involves complicated regulatory networks with multiple regulators controlling the expression of antibiotic biosynthetic pathways. One such regulatory network is that of the γ-butyrolactones, the so-called S. coelicolor butanolide (SCB) system. The γ-butyrolactones in this system serve as signalling molecules and bind to the receptor protein ScbR, releasing the repression of its target genes. The resulting expression changes affect the production of the two pigmented antibiotics Act and Red, as well as the transcription of the cpk antibiotic biosynthesis gene cluster and the synthesis of the γ-butyrolactones themselves. RESULTS: We identified a natural variant of ScbR in S. coelicolor (ScbR(M600)) that differs from ScbR in the genome-sequenced strain M145 (ScbR(M145)) by a single amino acid change, R120S. ScbR(M600) is impaired in its DNA binding ability and alters the expression of the pathway-specific regulatory genes of the red and cpk antibiotic biosynthesis gene clusters. Also, expression of the γ-butyrolactone biosynthesis gene scbA and production of the signalling molecules is slightly reduced. CONCLUSIONS: The γ-butyrolactone receptor, ScbR, plays a key role in the SCB regulatory cascade and in determining the onset of the expression of the antibiotic regulatory genes.
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spelling pubmed-34614102012-10-02 Characterisation of a natural variant of the γ-butyrolactone signalling receptor Gottelt, Marco Hesketh, Andrew Bunet, Robert Puri, Pranav Takano, Eriko BMC Res Notes Research Article BACKGROUND: The control of antibiotic production in Streptomyces coelicolor A3(2) involves complicated regulatory networks with multiple regulators controlling the expression of antibiotic biosynthetic pathways. One such regulatory network is that of the γ-butyrolactones, the so-called S. coelicolor butanolide (SCB) system. The γ-butyrolactones in this system serve as signalling molecules and bind to the receptor protein ScbR, releasing the repression of its target genes. The resulting expression changes affect the production of the two pigmented antibiotics Act and Red, as well as the transcription of the cpk antibiotic biosynthesis gene cluster and the synthesis of the γ-butyrolactones themselves. RESULTS: We identified a natural variant of ScbR in S. coelicolor (ScbR(M600)) that differs from ScbR in the genome-sequenced strain M145 (ScbR(M145)) by a single amino acid change, R120S. ScbR(M600) is impaired in its DNA binding ability and alters the expression of the pathway-specific regulatory genes of the red and cpk antibiotic biosynthesis gene clusters. Also, expression of the γ-butyrolactone biosynthesis gene scbA and production of the signalling molecules is slightly reduced. CONCLUSIONS: The γ-butyrolactone receptor, ScbR, plays a key role in the SCB regulatory cascade and in determining the onset of the expression of the antibiotic regulatory genes. BioMed Central 2012-07-27 /pmc/articles/PMC3461410/ /pubmed/22839725 http://dx.doi.org/10.1186/1756-0500-5-379 Text en Copyright ©2012 Gottelt et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Gottelt, Marco
Hesketh, Andrew
Bunet, Robert
Puri, Pranav
Takano, Eriko
Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title_full Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title_fullStr Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title_full_unstemmed Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title_short Characterisation of a natural variant of the γ-butyrolactone signalling receptor
title_sort characterisation of a natural variant of the γ-butyrolactone signalling receptor
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461410/
https://www.ncbi.nlm.nih.gov/pubmed/22839725
http://dx.doi.org/10.1186/1756-0500-5-379
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