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PML promotes MHC class II gene expression by stabilizing the class II transactivator
Promyelocytic leukemia (PML) nuclear bodies selectively associate with transcriptionally active genomic regions, including the gene-rich major histocompatibility (MHC) locus. In this paper, we have explored potential links between PML and interferon (IFN)-γ–induced MHC class II expression. IFN-γ ind...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461510/ https://www.ncbi.nlm.nih.gov/pubmed/23007646 http://dx.doi.org/10.1083/jcb.201112015 |
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author | Ulbricht, Tobias Alzrigat, Mohammad Horch, Almut Reuter, Nina von Mikecz, Anna Steimle, Viktor Schmitt, Eberhard Krämer, Oliver H. Stamminger, Thomas Hemmerich, Peter |
author_facet | Ulbricht, Tobias Alzrigat, Mohammad Horch, Almut Reuter, Nina von Mikecz, Anna Steimle, Viktor Schmitt, Eberhard Krämer, Oliver H. Stamminger, Thomas Hemmerich, Peter |
author_sort | Ulbricht, Tobias |
collection | PubMed |
description | Promyelocytic leukemia (PML) nuclear bodies selectively associate with transcriptionally active genomic regions, including the gene-rich major histocompatibility (MHC) locus. In this paper, we have explored potential links between PML and interferon (IFN)-γ–induced MHC class II expression. IFN-γ induced a substantial increase in the spatial proximity between PML bodies and the MHC class II gene cluster in different human cell types. Knockdown experiments show that PML is required for efficient IFN-γ–induced MHC II gene transcription through regulation of the class II transactivator (CIITA). PML mediates this function through protection of CIITA from proteasomal degradation. We also show that PML isoform II specifically forms a stable complex with CIITA at PML bodies. These observations establish PML as a coregulator of IFN-γ–induced MHC class II expression. |
format | Online Article Text |
id | pubmed-3461510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-34615102013-04-01 PML promotes MHC class II gene expression by stabilizing the class II transactivator Ulbricht, Tobias Alzrigat, Mohammad Horch, Almut Reuter, Nina von Mikecz, Anna Steimle, Viktor Schmitt, Eberhard Krämer, Oliver H. Stamminger, Thomas Hemmerich, Peter J Cell Biol Research Articles Promyelocytic leukemia (PML) nuclear bodies selectively associate with transcriptionally active genomic regions, including the gene-rich major histocompatibility (MHC) locus. In this paper, we have explored potential links between PML and interferon (IFN)-γ–induced MHC class II expression. IFN-γ induced a substantial increase in the spatial proximity between PML bodies and the MHC class II gene cluster in different human cell types. Knockdown experiments show that PML is required for efficient IFN-γ–induced MHC II gene transcription through regulation of the class II transactivator (CIITA). PML mediates this function through protection of CIITA from proteasomal degradation. We also show that PML isoform II specifically forms a stable complex with CIITA at PML bodies. These observations establish PML as a coregulator of IFN-γ–induced MHC class II expression. The Rockefeller University Press 2012-10-01 /pmc/articles/PMC3461510/ /pubmed/23007646 http://dx.doi.org/10.1083/jcb.201112015 Text en © 2012 Ulbricht et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Ulbricht, Tobias Alzrigat, Mohammad Horch, Almut Reuter, Nina von Mikecz, Anna Steimle, Viktor Schmitt, Eberhard Krämer, Oliver H. Stamminger, Thomas Hemmerich, Peter PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title | PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title_full | PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title_fullStr | PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title_full_unstemmed | PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title_short | PML promotes MHC class II gene expression by stabilizing the class II transactivator |
title_sort | pml promotes mhc class ii gene expression by stabilizing the class ii transactivator |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461510/ https://www.ncbi.nlm.nih.gov/pubmed/23007646 http://dx.doi.org/10.1083/jcb.201112015 |
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