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Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template

Background. An important step in the analysis of positron emission tomography (PET) studies of the brain is the definition of regions of interest (ROI). Image coregistration, ROI analysis, and quantification of brain PET data in small animals can be observer dependent. The purpose of this study was...

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Autores principales: Buiter, Hans J. C., van Velden, Floris H. P., Leysen, Josée E., Fisher, Abraham, Windhorst, Albert D., Lammertsma, Adriaan A., Huisman, Marc C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461609/
https://www.ncbi.nlm.nih.gov/pubmed/23050141
http://dx.doi.org/10.1155/2012/580717
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author Buiter, Hans J. C.
van Velden, Floris H. P.
Leysen, Josée E.
Fisher, Abraham
Windhorst, Albert D.
Lammertsma, Adriaan A.
Huisman, Marc C.
author_facet Buiter, Hans J. C.
van Velden, Floris H. P.
Leysen, Josée E.
Fisher, Abraham
Windhorst, Albert D.
Lammertsma, Adriaan A.
Huisman, Marc C.
author_sort Buiter, Hans J. C.
collection PubMed
description Background. An important step in the analysis of positron emission tomography (PET) studies of the brain is the definition of regions of interest (ROI). Image coregistration, ROI analysis, and quantification of brain PET data in small animals can be observer dependent. The purpose of this study was to investigate the feasibility of ROI analysis based on a standard MR template and an additional [(18)F]NaF scan. Methods. [(18)F]NaF scans of 10 Wistar rats were coregistered with a standard MR template by 3 observers and derived transformation matrices were applied to corresponding [(11)C]AF150(S) images. Uptake measures were derived for several brain regions delineated using the MR template. Overall agreement between the 3 observers was assessed by interclass correlation coefficients (ICC) of uptake data. In addition, [(11)C]AF150(S) ROI data were compared with ex vivo biodistribution data. Results. For all brain regions, ICC analysis showed excellent agreement between observers. Reproducibility, estimated by calculation of standard deviation of the between-observer differences, was demonstrated by an average of 17% expressed as coefficient of variation. Uptake of [(11)C]AF150(S) derived from ROI analysis closely matched ex vivo biodistribution data. Conclusions. The proposed method provides a reproducible and tracer-independent method for ROI analysis of rat brain PET data.
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spelling pubmed-34616092012-10-04 Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template Buiter, Hans J. C. van Velden, Floris H. P. Leysen, Josée E. Fisher, Abraham Windhorst, Albert D. Lammertsma, Adriaan A. Huisman, Marc C. Int J Mol Imaging Research Article Background. An important step in the analysis of positron emission tomography (PET) studies of the brain is the definition of regions of interest (ROI). Image coregistration, ROI analysis, and quantification of brain PET data in small animals can be observer dependent. The purpose of this study was to investigate the feasibility of ROI analysis based on a standard MR template and an additional [(18)F]NaF scan. Methods. [(18)F]NaF scans of 10 Wistar rats were coregistered with a standard MR template by 3 observers and derived transformation matrices were applied to corresponding [(11)C]AF150(S) images. Uptake measures were derived for several brain regions delineated using the MR template. Overall agreement between the 3 observers was assessed by interclass correlation coefficients (ICC) of uptake data. In addition, [(11)C]AF150(S) ROI data were compared with ex vivo biodistribution data. Results. For all brain regions, ICC analysis showed excellent agreement between observers. Reproducibility, estimated by calculation of standard deviation of the between-observer differences, was demonstrated by an average of 17% expressed as coefficient of variation. Uptake of [(11)C]AF150(S) derived from ROI analysis closely matched ex vivo biodistribution data. Conclusions. The proposed method provides a reproducible and tracer-independent method for ROI analysis of rat brain PET data. Hindawi Publishing Corporation 2012 2012-09-23 /pmc/articles/PMC3461609/ /pubmed/23050141 http://dx.doi.org/10.1155/2012/580717 Text en Copyright © 2012 Hans J. C. Buiter et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Buiter, Hans J. C.
van Velden, Floris H. P.
Leysen, Josée E.
Fisher, Abraham
Windhorst, Albert D.
Lammertsma, Adriaan A.
Huisman, Marc C.
Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title_full Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title_fullStr Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title_full_unstemmed Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title_short Reproducible Analysis of Rat Brain PET Studies Using an Additional [(18)F]NaF Scan and an MR-Based ROI Template
title_sort reproducible analysis of rat brain pet studies using an additional [(18)f]naf scan and an mr-based roi template
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3461609/
https://www.ncbi.nlm.nih.gov/pubmed/23050141
http://dx.doi.org/10.1155/2012/580717
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