Cargando…

Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation

BACKGROUND: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Yulian, Zacur, Howard, Cheadle, Chris, Ning, Ning, Fan, Jinshui, Vlahos, Nikos F
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462109/
https://www.ncbi.nlm.nih.gov/pubmed/22950660
http://dx.doi.org/10.1186/1477-7827-10-72
_version_ 1782245138587713536
author Zhao, Yulian
Zacur, Howard
Cheadle, Chris
Ning, Ning
Fan, Jinshui
Vlahos, Nikos F
author_facet Zhao, Yulian
Zacur, Howard
Cheadle, Chris
Ning, Ning
Fan, Jinshui
Vlahos, Nikos F
author_sort Zhao, Yulian
collection PubMed
description BACKGROUND: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles. METHODS: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3–5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel. RESULTS: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p < 0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3–5 days after retrieval. During the peri-implantation period (3–5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group. CONCLUSION: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes.
format Online
Article
Text
id pubmed-3462109
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-34621092012-10-02 Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation Zhao, Yulian Zacur, Howard Cheadle, Chris Ning, Ning Fan, Jinshui Vlahos, Nikos F Reprod Biol Endocrinol Research BACKGROUND: Studies suggested that microRNAs influence cellular activities in the uterus including cell differentiation and embryo implantation. In assisted reproduction cycles, luteal phase support, given to improve endometrial characteristics and to facilitate the implantation process, has been a standard practice. The effect of different types of luteal phase support using steroid hormones in relation to endometrial miRNA profiles during the peri-implantation period has not seen described. This study was designed to evaluate the expression of miRNAs during the luteal phase following controlled ovarian stimulation for IVF and the influence of different luteal phase support protocols on miRNA profiles. METHODS: The study was approved by the Johns Hopkins Hospital Institutional Review Board. Endometrial biopsies were obtained on the day of oocyte retrieval from 9 oocyte donors (group I). An additional endometrial biopsy was obtained 3–5 days later (Group II) after the donors were randomized into three groups. Group IIa had no luteal-phase support, group IIb had luteal support with micronized progesterone (P), and Group IIc had luteal support with progesterone plus 17-beta-estradiol (P + E). Total RNA was isolated and microarray analysis was performed using an Illumina miRNA expression panel. RESULTS: A total of 526 miRNAs were identified. Out of those, 216 miRNAs were differentially regulated (p < 0.05) between the comparison groups. As compared to the day of retrieval, 19, 11 and 6 miRNAs were differentially regulated more than 2 fold in the groups of no support, in the P support only, and in the P + E support respectively, 3–5 days after retrieval. During the peri-implantation period (3–5 days after retrieval) the expression of 33 and 6 miRNAs increased, while the expression of 3 and 0 miRNAs decreased, in the P alone and in the P + E group respectively as compared to the no steroid supplementation group. CONCLUSION: Luteal support following COS has a profound influence on miRNA profiles. Up or down regulation of miRNAs after P or P + E support suggest a role(s) of luteal support in the peri-implantation uterus in IVF cycles through the regulation of associated target genes. BioMed Central 2012-09-06 /pmc/articles/PMC3462109/ /pubmed/22950660 http://dx.doi.org/10.1186/1477-7827-10-72 Text en Copyright ©2012 Zhao et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Zhao, Yulian
Zacur, Howard
Cheadle, Chris
Ning, Ning
Fan, Jinshui
Vlahos, Nikos F
Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title_full Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title_fullStr Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title_full_unstemmed Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title_short Effect of luteal-phase support on endometrial microRNA expression following controlled ovarian stimulation
title_sort effect of luteal-phase support on endometrial microrna expression following controlled ovarian stimulation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462109/
https://www.ncbi.nlm.nih.gov/pubmed/22950660
http://dx.doi.org/10.1186/1477-7827-10-72
work_keys_str_mv AT zhaoyulian effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation
AT zacurhoward effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation
AT cheadlechris effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation
AT ningning effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation
AT fanjinshui effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation
AT vlahosnikosf effectoflutealphasesupportonendometrialmicrornaexpressionfollowingcontrolledovarianstimulation