Cargando…
Polymer Microarrays for High Throughput Discovery of Biomaterials
The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format(1). Herein is described the formation and characterizatio...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MyJove Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462568/ https://www.ncbi.nlm.nih.gov/pubmed/22314927 http://dx.doi.org/10.3791/3636 |
_version_ | 1782245183137513472 |
---|---|
author | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Langer, Robert Anderson, Daniel G. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. |
author_facet | Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Langer, Robert Anderson, Daniel G. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. |
author_sort | Hook, Andrew L. |
collection | PubMed |
description | The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format(1). Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique (2). This involves mixing monomers at varied ratios to produce a library of monomer solutions, transferring the solution to a glass slide format using a robotic printing device and curing with UV irradiation. This format is readily amenable to many biological assays, including stem cell attachment and proliferation, cell sorting and low bacterial adhesion, allowing the ready identification of 'hit' materials that fulfill a specific biological criterion(3-5). Furthermore, the use of high throughput surface characterization (HTSC) allows the biological performance to be correlated with physio-chemical properties, hence elucidating the biological-material interaction(6). HTSC makes use of water contact angle (WCA) measurements, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). In particular, ToF-SIMS provides a chemically rich analysis of the sample that can be used to correlate the cell response with a molecular moiety. In some cases, the biological performance can be predicted from the ToF-SIMS spectra, demonstrating the chemical dependence of a biological-material interaction, and informing the development of hit materials(5,3). |
format | Online Article Text |
id | pubmed-3462568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | MyJove Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34625682012-10-05 Polymer Microarrays for High Throughput Discovery of Biomaterials Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Langer, Robert Anderson, Daniel G. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. J Vis Exp Bioengineering The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format(1). Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique (2). This involves mixing monomers at varied ratios to produce a library of monomer solutions, transferring the solution to a glass slide format using a robotic printing device and curing with UV irradiation. This format is readily amenable to many biological assays, including stem cell attachment and proliferation, cell sorting and low bacterial adhesion, allowing the ready identification of 'hit' materials that fulfill a specific biological criterion(3-5). Furthermore, the use of high throughput surface characterization (HTSC) allows the biological performance to be correlated with physio-chemical properties, hence elucidating the biological-material interaction(6). HTSC makes use of water contact angle (WCA) measurements, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). In particular, ToF-SIMS provides a chemically rich analysis of the sample that can be used to correlate the cell response with a molecular moiety. In some cases, the biological performance can be predicted from the ToF-SIMS spectra, demonstrating the chemical dependence of a biological-material interaction, and informing the development of hit materials(5,3). MyJove Corporation 2012-01-25 /pmc/articles/PMC3462568/ /pubmed/22314927 http://dx.doi.org/10.3791/3636 Text en Copyright © 2012, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/ |
spellingShingle | Bioengineering Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Langer, Robert Anderson, Daniel G. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R. Polymer Microarrays for High Throughput Discovery of Biomaterials |
title | Polymer Microarrays for High Throughput Discovery of Biomaterials |
title_full | Polymer Microarrays for High Throughput Discovery of Biomaterials |
title_fullStr | Polymer Microarrays for High Throughput Discovery of Biomaterials |
title_full_unstemmed | Polymer Microarrays for High Throughput Discovery of Biomaterials |
title_short | Polymer Microarrays for High Throughput Discovery of Biomaterials |
title_sort | polymer microarrays for high throughput discovery of biomaterials |
topic | Bioengineering |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462568/ https://www.ncbi.nlm.nih.gov/pubmed/22314927 http://dx.doi.org/10.3791/3636 |
work_keys_str_mv | AT hookandrewl polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT changchienyi polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT yangjing polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT scurrdavidj polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT langerrobert polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT andersondanielg polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT atkinsonsteve polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT williamspaul polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT daviesmartync polymermicroarraysforhighthroughputdiscoveryofbiomaterials AT alexandermorganr polymermicroarraysforhighthroughputdiscoveryofbiomaterials |