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Characterization of a set of novel meiotically-active promoters in Arabidopsis
BACKGROUND: Homologous recombination, together with selection, laid the foundation for traditional plant breeding. The recombination process that takes place during meiotic cell division is crucial for the creation of novel variations of highly desired traits by breeders. Gaining control over this p...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462685/ https://www.ncbi.nlm.nih.gov/pubmed/22776406 http://dx.doi.org/10.1186/1471-2229-12-104 |
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author | Li, Junhua Farmer, Andrew D Lindquist, Ingrid E Dukowic-Schulze, Stefanie Mudge, Joann Li, Tao Retzel, Ernest F Chen, Changbin |
author_facet | Li, Junhua Farmer, Andrew D Lindquist, Ingrid E Dukowic-Schulze, Stefanie Mudge, Joann Li, Tao Retzel, Ernest F Chen, Changbin |
author_sort | Li, Junhua |
collection | PubMed |
description | BACKGROUND: Homologous recombination, together with selection, laid the foundation for traditional plant breeding. The recombination process that takes place during meiotic cell division is crucial for the creation of novel variations of highly desired traits by breeders. Gaining control over this process is important for molecular breeding to achieve more precise, large-scale and quicker plant improvement. As conventional ubiquitous promoters are neither tissue-specific nor efficient in driving gene expression in meiocytes, promoters with high meiotic activities are potential candidates for manipulating the recombination process. So far, only a few meiotically-active promoters have been reported. Recently developed techniques to profile the transcriptome landscape of isolated meiocytes provided the means to discover promoters from genes that are actively expressed in meiosis. RESULTS: In a screen for meiotically-active promoters, we examined ten promoter sequences that are associated with novel meiotic candidate genes. Each promoter was tested by expressing a GFP reporter gene in Arabidopsis. Characterization of regulatory regions revealed that these meiotically-active promoters possessed conserved motifs and motif arrangement. Some of the promoters unite optimal properties which are invaluable for meiosis-directed studies such as delivering specific gene expression in early meiosis I and/or meiosis II. Furthermore, the examination of homologs of the corresponding genes within green plants points to a great potential of applying the information from Arabidopsis to other species, especially crop plants. CONCLUSIONS: We identified ten novel meiotically-active promoters; which, along with their homologs, are prime candidates to specifically drive gene expression during meiosis in plants and can thus provide important tools for meiosis study and crop breeding. |
format | Online Article Text |
id | pubmed-3462685 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34626852012-10-03 Characterization of a set of novel meiotically-active promoters in Arabidopsis Li, Junhua Farmer, Andrew D Lindquist, Ingrid E Dukowic-Schulze, Stefanie Mudge, Joann Li, Tao Retzel, Ernest F Chen, Changbin BMC Plant Biol Research Article BACKGROUND: Homologous recombination, together with selection, laid the foundation for traditional plant breeding. The recombination process that takes place during meiotic cell division is crucial for the creation of novel variations of highly desired traits by breeders. Gaining control over this process is important for molecular breeding to achieve more precise, large-scale and quicker plant improvement. As conventional ubiquitous promoters are neither tissue-specific nor efficient in driving gene expression in meiocytes, promoters with high meiotic activities are potential candidates for manipulating the recombination process. So far, only a few meiotically-active promoters have been reported. Recently developed techniques to profile the transcriptome landscape of isolated meiocytes provided the means to discover promoters from genes that are actively expressed in meiosis. RESULTS: In a screen for meiotically-active promoters, we examined ten promoter sequences that are associated with novel meiotic candidate genes. Each promoter was tested by expressing a GFP reporter gene in Arabidopsis. Characterization of regulatory regions revealed that these meiotically-active promoters possessed conserved motifs and motif arrangement. Some of the promoters unite optimal properties which are invaluable for meiosis-directed studies such as delivering specific gene expression in early meiosis I and/or meiosis II. Furthermore, the examination of homologs of the corresponding genes within green plants points to a great potential of applying the information from Arabidopsis to other species, especially crop plants. CONCLUSIONS: We identified ten novel meiotically-active promoters; which, along with their homologs, are prime candidates to specifically drive gene expression during meiosis in plants and can thus provide important tools for meiosis study and crop breeding. BioMed Central 2012-07-09 /pmc/articles/PMC3462685/ /pubmed/22776406 http://dx.doi.org/10.1186/1471-2229-12-104 Text en Copyright ©2012 Li et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Junhua Farmer, Andrew D Lindquist, Ingrid E Dukowic-Schulze, Stefanie Mudge, Joann Li, Tao Retzel, Ernest F Chen, Changbin Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title | Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title_full | Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title_fullStr | Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title_full_unstemmed | Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title_short | Characterization of a set of novel meiotically-active promoters in Arabidopsis |
title_sort | characterization of a set of novel meiotically-active promoters in arabidopsis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462685/ https://www.ncbi.nlm.nih.gov/pubmed/22776406 http://dx.doi.org/10.1186/1471-2229-12-104 |
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