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Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts
BACKGROUND: Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462690/ https://www.ncbi.nlm.nih.gov/pubmed/22974481 http://dx.doi.org/10.1186/1472-6882-12-153 |
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author | Chaabane, Fadwa Boubaker, Jihed Loussaif, Amira Neffati, Aicha Kilani-Jaziri, Somaya Ghedira, Kamel Chekir-Ghedira, Leila |
author_facet | Chaabane, Fadwa Boubaker, Jihed Loussaif, Amira Neffati, Aicha Kilani-Jaziri, Somaya Ghedira, Kamel Chekir-Ghedira, Leila |
author_sort | Chaabane, Fadwa |
collection | PubMed |
description | BACKGROUND: Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. METHODS: The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF) enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. RESULTS: None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. CONCLUSIONS: The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules. |
format | Online Article Text |
id | pubmed-3462690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34626902012-10-03 Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts Chaabane, Fadwa Boubaker, Jihed Loussaif, Amira Neffati, Aicha Kilani-Jaziri, Somaya Ghedira, Kamel Chekir-Ghedira, Leila BMC Complement Altern Med Research Article BACKGROUND: Plants play a significant role in maintaining human health and improving the quality of human life. They serve humans well as valuable components of food, as well as in cosmetics, dyes, and medicines. In fact, many plant extracts prepared from plants have been shown to exert biological activity in vitro and in vivo. The present study explored antioxidant and antigenotoxic effects of Daphne gnidium leaf extracts. METHODS: The genotoxic potential of petroleum ether, chloroform, ethyl acetate, methanol and total oligomer flavonoid (TOF) enriched extracts from leaves of Daphne gnidium, was assessed using Escherichia coli PQ37. Likewise, the antigenotoxicity of the same extracts was tested using the “SOS chromotest test”. Antioxidant activities were studied using non enzymatic and enzymatic method: NBT/Riboflavine and xantine oxidase. RESULTS: None of the different extracts produced a genotoxic effect, except TOF extract at the lowest tested dose. Our results showed that D. gnidium leaf extracts possess an antigenotoxic effect against the nitrofurantoin a mutagen of reference. Ethyl acetate and TOF extracts were the most effective in inhibiting xanthine oxidase activity. While, methanol extract was the most potent superoxide scavenger when tested with the NBT/Riboflavine assay. CONCLUSIONS: The present study has demonstrated that D. gnidium leaf extract possess antioxidant and antigenotoxic effects. These activities could be ascribed to compounds like polyphenols and flavonoid. Further studies are required to isolate the active molecules. BioMed Central 2012-09-13 /pmc/articles/PMC3462690/ /pubmed/22974481 http://dx.doi.org/10.1186/1472-6882-12-153 Text en Copyright ©2012 Chaabane et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Chaabane, Fadwa Boubaker, Jihed Loussaif, Amira Neffati, Aicha Kilani-Jaziri, Somaya Ghedira, Kamel Chekir-Ghedira, Leila Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title | Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title_full | Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title_fullStr | Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title_full_unstemmed | Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title_short | Antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
title_sort | antioxidant, genotoxic and antigenotoxic activities of daphne gnidium leaf extracts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462690/ https://www.ncbi.nlm.nih.gov/pubmed/22974481 http://dx.doi.org/10.1186/1472-6882-12-153 |
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