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A cost-effective method for Illumina small RNA-Seq library preparation using T4 RNA ligase 1 adenylated adapters

BACKGROUND: Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety. In the absence of ATP, this adapter can be ligated to the 3’ hydroxyl group of sma...

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Detalles Bibliográficos
Autores principales:	Chen, Yun-Ru, Zheng, Yi, Liu, Bao, Zhong, Silin, Giovannoni, Jim, Fei, Zhangjun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462708/ https://www.ncbi.nlm.nih.gov/pubmed/22995534 http://dx.doi.org/10.1186/1746-4811-8-41

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