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Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle

Transplantation of muscle precursor cells is of therapeutic interest for focal skeletal muscular diseases. However, major limitations of cell transplantation are the poor survival, expansion and migration of the injected cells. The massive and early death of transplanted myoblasts is not fully under...

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Autores principales: Lesault, Pierre-François, Theret, Marine, Magnan, Mélanie, Cuvellier, Sylvain, Niu, Yiming, Gherardi, Romain K., Tremblay, Jacques P., Hittinger, Luc, Chazaud, Bénédicte
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462747/
https://www.ncbi.nlm.nih.gov/pubmed/23056408
http://dx.doi.org/10.1371/journal.pone.0046698
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author Lesault, Pierre-François
Theret, Marine
Magnan, Mélanie
Cuvellier, Sylvain
Niu, Yiming
Gherardi, Romain K.
Tremblay, Jacques P.
Hittinger, Luc
Chazaud, Bénédicte
author_facet Lesault, Pierre-François
Theret, Marine
Magnan, Mélanie
Cuvellier, Sylvain
Niu, Yiming
Gherardi, Romain K.
Tremblay, Jacques P.
Hittinger, Luc
Chazaud, Bénédicte
author_sort Lesault, Pierre-François
collection PubMed
description Transplantation of muscle precursor cells is of therapeutic interest for focal skeletal muscular diseases. However, major limitations of cell transplantation are the poor survival, expansion and migration of the injected cells. The massive and early death of transplanted myoblasts is not fully understood although several mechanisms have been suggested. Various attempts have been made to improve their survival or migration. Taking into account that muscle regeneration is associated with the presence of macrophages, which are helpful in repairing the muscle by both cleansing the debris and deliver trophic cues to myoblasts in a sequential way, we attempted in the present work to improve myoblast transplantation by coinjecting macrophages. The present data showed that in the 5 days following the transplantation, macrophages efficiently improved: i) myoblast survival by limiting their massive death, ii) myoblast expansion within the tissue and iii) myoblast migration in the dystrophic muscle. This was confirmed by in vitro analyses showing that macrophages stimulated myoblast adhesion and migration. As a result, myoblast contribution to regenerating host myofibres was increased by macrophages one month after transplantation. Altogether, these data demonstrate that macrophages are beneficial during the early steps of myoblast transplantation into skeletal muscle, showing that coinjecting these stromal cells may be used as a helper to improve the efficiency of parenchymal cell engraftment.
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spelling pubmed-34627472012-10-10 Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle Lesault, Pierre-François Theret, Marine Magnan, Mélanie Cuvellier, Sylvain Niu, Yiming Gherardi, Romain K. Tremblay, Jacques P. Hittinger, Luc Chazaud, Bénédicte PLoS One Research Article Transplantation of muscle precursor cells is of therapeutic interest for focal skeletal muscular diseases. However, major limitations of cell transplantation are the poor survival, expansion and migration of the injected cells. The massive and early death of transplanted myoblasts is not fully understood although several mechanisms have been suggested. Various attempts have been made to improve their survival or migration. Taking into account that muscle regeneration is associated with the presence of macrophages, which are helpful in repairing the muscle by both cleansing the debris and deliver trophic cues to myoblasts in a sequential way, we attempted in the present work to improve myoblast transplantation by coinjecting macrophages. The present data showed that in the 5 days following the transplantation, macrophages efficiently improved: i) myoblast survival by limiting their massive death, ii) myoblast expansion within the tissue and iii) myoblast migration in the dystrophic muscle. This was confirmed by in vitro analyses showing that macrophages stimulated myoblast adhesion and migration. As a result, myoblast contribution to regenerating host myofibres was increased by macrophages one month after transplantation. Altogether, these data demonstrate that macrophages are beneficial during the early steps of myoblast transplantation into skeletal muscle, showing that coinjecting these stromal cells may be used as a helper to improve the efficiency of parenchymal cell engraftment. Public Library of Science 2012-10-02 /pmc/articles/PMC3462747/ /pubmed/23056408 http://dx.doi.org/10.1371/journal.pone.0046698 Text en © 2012 Lesault et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lesault, Pierre-François
Theret, Marine
Magnan, Mélanie
Cuvellier, Sylvain
Niu, Yiming
Gherardi, Romain K.
Tremblay, Jacques P.
Hittinger, Luc
Chazaud, Bénédicte
Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title_full Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title_fullStr Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title_full_unstemmed Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title_short Macrophages Improve Survival, Proliferation and Migration of Engrafted Myogenic Precursor Cells into MDX Skeletal Muscle
title_sort macrophages improve survival, proliferation and migration of engrafted myogenic precursor cells into mdx skeletal muscle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3462747/
https://www.ncbi.nlm.nih.gov/pubmed/23056408
http://dx.doi.org/10.1371/journal.pone.0046698
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