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Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group
Purpose. (123)I-metaiodobenzylguanidine (MIBG) is used for the diagnostic evaluation of neuroblastoma. We evaluated the relationship between norepinephrine transporter (NET) expression and clinical MIBG uptake. Methods. Quantitative reverse transcription PCR (N = 82) and immunohistochemistry (IHC; N...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463166/ https://www.ncbi.nlm.nih.gov/pubmed/23050139 http://dx.doi.org/10.1155/2012/250834 |
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author | DuBois, Steven G. Geier, Ethan Batra, Vandana Yee, Sook Wah Neuhaus, John Segal, Mark Martinez, Daniel Pawel, Bruce Yanik, Greg Naranjo, Arlene London, Wendy B. Kreissman, Susan Baker, David Attiyeh, Edward Hogarty, Michael D. Maris, John M. Giacomini, Kathleen Matthay, Katherine K. |
author_facet | DuBois, Steven G. Geier, Ethan Batra, Vandana Yee, Sook Wah Neuhaus, John Segal, Mark Martinez, Daniel Pawel, Bruce Yanik, Greg Naranjo, Arlene London, Wendy B. Kreissman, Susan Baker, David Attiyeh, Edward Hogarty, Michael D. Maris, John M. Giacomini, Kathleen Matthay, Katherine K. |
author_sort | DuBois, Steven G. |
collection | PubMed |
description | Purpose. (123)I-metaiodobenzylguanidine (MIBG) is used for the diagnostic evaluation of neuroblastoma. We evaluated the relationship between norepinephrine transporter (NET) expression and clinical MIBG uptake. Methods. Quantitative reverse transcription PCR (N = 82) and immunohistochemistry (IHC; N = 61) were performed for neuroblastoma NET mRNA and protein expression and correlated with MIBG avidity on diagnostic scans. The correlation of NET expression with clinical features was also performed. Results. Median NET mRNA expression level for the 19 MIBG avid patients was 12.9% (range 1.6–73.7%) versus 5.9% (range 0.6–110.0%) for the 8 nonavid patients (P = 0.31). Median percent NET protein expression was 50% (range 0–100%) in MIBG avid patients compared to 10% (range 0–80%) in nonavid patients (P = 0.027). MYCN amplified tumors had lower NET protein expression compared to nonamplified tumors (10% versus 50%; P = 0.0002). Conclusions. NET protein expression in neuroblastoma correlates with MIBG avidity. MYCN amplified tumors have lower NET protein expression. |
format | Online Article Text |
id | pubmed-3463166 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34631662012-10-04 Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group DuBois, Steven G. Geier, Ethan Batra, Vandana Yee, Sook Wah Neuhaus, John Segal, Mark Martinez, Daniel Pawel, Bruce Yanik, Greg Naranjo, Arlene London, Wendy B. Kreissman, Susan Baker, David Attiyeh, Edward Hogarty, Michael D. Maris, John M. Giacomini, Kathleen Matthay, Katherine K. Int J Mol Imaging Research Article Purpose. (123)I-metaiodobenzylguanidine (MIBG) is used for the diagnostic evaluation of neuroblastoma. We evaluated the relationship between norepinephrine transporter (NET) expression and clinical MIBG uptake. Methods. Quantitative reverse transcription PCR (N = 82) and immunohistochemistry (IHC; N = 61) were performed for neuroblastoma NET mRNA and protein expression and correlated with MIBG avidity on diagnostic scans. The correlation of NET expression with clinical features was also performed. Results. Median NET mRNA expression level for the 19 MIBG avid patients was 12.9% (range 1.6–73.7%) versus 5.9% (range 0.6–110.0%) for the 8 nonavid patients (P = 0.31). Median percent NET protein expression was 50% (range 0–100%) in MIBG avid patients compared to 10% (range 0–80%) in nonavid patients (P = 0.027). MYCN amplified tumors had lower NET protein expression compared to nonamplified tumors (10% versus 50%; P = 0.0002). Conclusions. NET protein expression in neuroblastoma correlates with MIBG avidity. MYCN amplified tumors have lower NET protein expression. Hindawi Publishing Corporation 2012 2012-09-25 /pmc/articles/PMC3463166/ /pubmed/23050139 http://dx.doi.org/10.1155/2012/250834 Text en Copyright © 2012 Steven G. DuBois et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article DuBois, Steven G. Geier, Ethan Batra, Vandana Yee, Sook Wah Neuhaus, John Segal, Mark Martinez, Daniel Pawel, Bruce Yanik, Greg Naranjo, Arlene London, Wendy B. Kreissman, Susan Baker, David Attiyeh, Edward Hogarty, Michael D. Maris, John M. Giacomini, Kathleen Matthay, Katherine K. Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title | Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title_full | Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title_fullStr | Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title_full_unstemmed | Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title_short | Evaluation of Norepinephrine Transporter Expression and Metaiodobenzylguanidine Avidity in Neuroblastoma: A Report from the Children's Oncology Group |
title_sort | evaluation of norepinephrine transporter expression and metaiodobenzylguanidine avidity in neuroblastoma: a report from the children's oncology group |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463166/ https://www.ncbi.nlm.nih.gov/pubmed/23050139 http://dx.doi.org/10.1155/2012/250834 |
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