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Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery

PURPOSE: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages of copolymers and silica, studies on the preparation of copolymer-silica composites and cellular evaluation were carried out. METHO...

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Autores principales: Yuan, Hong, Bao, Xin, Du, Yong-Zhong, You, Jian, Hu, Fu-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463401/
https://www.ncbi.nlm.nih.gov/pubmed/23055724
http://dx.doi.org/10.2147/IJN.S35575
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author Yuan, Hong
Bao, Xin
Du, Yong-Zhong
You, Jian
Hu, Fu-Qiang
author_facet Yuan, Hong
Bao, Xin
Du, Yong-Zhong
You, Jian
Hu, Fu-Qiang
author_sort Yuan, Hong
collection PubMed
description PURPOSE: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages of copolymers and silica, studies on the preparation of copolymer-silica composites and cellular evaluation were carried out. METHODS: First, a stearic acid-g-chitosan (CS-SA) copolymer was synthesized through a coupling reaction, and then silicone oxide (SiO(2))-deposited doxorubicin (DOX)-loaded stearic acid-g-chitosan (CS-SA/SiO(2)/DOX) nanoparticles were prepared through the sol-gel reaction. Physical and chemical properties such as particle size, zeta potential, and morphologies were examined, and small-angle X-ray scattering (SAXS) analysis was employed to identify the mesoporous structures of the generated nanoparticles. Cellular uptake and cytotoxicity studies were also conducted. RESULTS: CS-SA/SiO(2)/DOX nanoparticles with different amounts of SiO(2) deposited were obtained, and SAXS studies showed that mesoporous structures existed in the CS-SA/SiO(2)/DOX nanoparticles. The mesoporous size of middle-ratio and high-ratio deposited CS-SA/SiO(2)/DOX nanoparticles were 4–5 nm and 8–10 nm, respectively. Based on transmission electron microscopy images of CS-SA/SiO(2)/DOX nanoparticles, dark rings around the nanoparticles could be observed in contrast with CS-SA/DOX micelles. Furthermore, CS-SA/SiO(2)/DOX nanoparticles exhibited faster release behavior in vitro than CS-SA/DOX micelles; cellular uptake research in A549 indicated that the CS-SA/SiO(2)/DOX nanoparticles were taken up by A549 cells more rapidly, and that CS-SA/SiO(2)/DOX nanoparticles entered the cell more easily when the amount of SiO(2) was higher. IC(50) values of CS-SA/DOX micelles, CS-SA/SiO(2)/DOX-4, CS-SA/SiO(2)/DOX-8, and CS-SA/SiO(2)/DOX-16 nanoparticles against A549 cells measured using the MTT assay were 1.69, 0.93, 0.32, and 0.12 μg/mL, respectively. CONCLUSION: SiO(2)-deposited stearic acid-g-chitosan organic–inorganic composites show promise as nanocarriers for hydrophobic drugs such as DOX.
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spelling pubmed-34634012012-10-09 Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery Yuan, Hong Bao, Xin Du, Yong-Zhong You, Jian Hu, Fu-Qiang Int J Nanomedicine Original Research PURPOSE: Both polymer micelles and mesoporous silica nanoparticles have been widely researched as vectors for small molecular insoluble drugs. To combine the advantages of copolymers and silica, studies on the preparation of copolymer-silica composites and cellular evaluation were carried out. METHODS: First, a stearic acid-g-chitosan (CS-SA) copolymer was synthesized through a coupling reaction, and then silicone oxide (SiO(2))-deposited doxorubicin (DOX)-loaded stearic acid-g-chitosan (CS-SA/SiO(2)/DOX) nanoparticles were prepared through the sol-gel reaction. Physical and chemical properties such as particle size, zeta potential, and morphologies were examined, and small-angle X-ray scattering (SAXS) analysis was employed to identify the mesoporous structures of the generated nanoparticles. Cellular uptake and cytotoxicity studies were also conducted. RESULTS: CS-SA/SiO(2)/DOX nanoparticles with different amounts of SiO(2) deposited were obtained, and SAXS studies showed that mesoporous structures existed in the CS-SA/SiO(2)/DOX nanoparticles. The mesoporous size of middle-ratio and high-ratio deposited CS-SA/SiO(2)/DOX nanoparticles were 4–5 nm and 8–10 nm, respectively. Based on transmission electron microscopy images of CS-SA/SiO(2)/DOX nanoparticles, dark rings around the nanoparticles could be observed in contrast with CS-SA/DOX micelles. Furthermore, CS-SA/SiO(2)/DOX nanoparticles exhibited faster release behavior in vitro than CS-SA/DOX micelles; cellular uptake research in A549 indicated that the CS-SA/SiO(2)/DOX nanoparticles were taken up by A549 cells more rapidly, and that CS-SA/SiO(2)/DOX nanoparticles entered the cell more easily when the amount of SiO(2) was higher. IC(50) values of CS-SA/DOX micelles, CS-SA/SiO(2)/DOX-4, CS-SA/SiO(2)/DOX-8, and CS-SA/SiO(2)/DOX-16 nanoparticles against A549 cells measured using the MTT assay were 1.69, 0.93, 0.32, and 0.12 μg/mL, respectively. CONCLUSION: SiO(2)-deposited stearic acid-g-chitosan organic–inorganic composites show promise as nanocarriers for hydrophobic drugs such as DOX. Dove Medical Press 2012 2012-09-26 /pmc/articles/PMC3463401/ /pubmed/23055724 http://dx.doi.org/10.2147/IJN.S35575 Text en © 2012 Yuan et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Yuan, Hong
Bao, Xin
Du, Yong-Zhong
You, Jian
Hu, Fu-Qiang
Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_full Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_fullStr Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_full_unstemmed Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_short Preparation and evaluation of SiO(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
title_sort preparation and evaluation of sio(2)-deposited stearic acid-g-chitosan nanoparticles for doxorubicin delivery
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463401/
https://www.ncbi.nlm.nih.gov/pubmed/23055724
http://dx.doi.org/10.2147/IJN.S35575
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