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In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mech...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463435/ https://www.ncbi.nlm.nih.gov/pubmed/22925563 http://dx.doi.org/10.1186/1743-422X-9-175 |
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author | Feng, Li Li, Xiao-Quan Li, Xiao-ning Li, Jun Meng, Xian-Ming Zhang, Hong-Yun Liang, Jing-Jing Li, Hui Sun, Shi-Kai Cai, Xin-Bin Su, Li-Juan Yin, Shan Li, Yan-Sheng Luo, Ting Rong |
author_facet | Feng, Li Li, Xiao-Quan Li, Xiao-ning Li, Jun Meng, Xian-Ming Zhang, Hong-Yun Liang, Jing-Jing Li, Hui Sun, Shi-Kai Cai, Xin-Bin Su, Li-Juan Yin, Shan Li, Yan-Sheng Luo, Ting Rong |
author_sort | Feng, Li |
collection | PubMed |
description | BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear. RESULTS: In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001). CONCLUSION: We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF. |
format | Online Article Text |
id | pubmed-3463435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-34634352012-10-04 In vitro infection with classical swine fever virus inhibits the transcription of immune response genes Feng, Li Li, Xiao-Quan Li, Xiao-ning Li, Jun Meng, Xian-Ming Zhang, Hong-Yun Liang, Jing-Jing Li, Hui Sun, Shi-Kai Cai, Xin-Bin Su, Li-Juan Yin, Shan Li, Yan-Sheng Luo, Ting Rong Virol J Research BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear. RESULTS: In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001). CONCLUSION: We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF. BioMed Central 2012-08-28 /pmc/articles/PMC3463435/ /pubmed/22925563 http://dx.doi.org/10.1186/1743-422X-9-175 Text en Copyright ©2012 Feng et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Feng, Li Li, Xiao-Quan Li, Xiao-ning Li, Jun Meng, Xian-Ming Zhang, Hong-Yun Liang, Jing-Jing Li, Hui Sun, Shi-Kai Cai, Xin-Bin Su, Li-Juan Yin, Shan Li, Yan-Sheng Luo, Ting Rong In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title | In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title_full | In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title_fullStr | In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title_full_unstemmed | In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title_short | In vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
title_sort | in vitro infection with classical swine fever virus inhibits the transcription of immune response genes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463435/ https://www.ncbi.nlm.nih.gov/pubmed/22925563 http://dx.doi.org/10.1186/1743-422X-9-175 |
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