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In vitro infection with classical swine fever virus inhibits the transcription of immune response genes

BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mech...

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Autores principales: Feng, Li, Li, Xiao-Quan, Li, Xiao-ning, Li, Jun, Meng, Xian-Ming, Zhang, Hong-Yun, Liang, Jing-Jing, Li, Hui, Sun, Shi-Kai, Cai, Xin-Bin, Su, Li-Juan, Yin, Shan, Li, Yan-Sheng, Luo, Ting Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463435/
https://www.ncbi.nlm.nih.gov/pubmed/22925563
http://dx.doi.org/10.1186/1743-422X-9-175
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author Feng, Li
Li, Xiao-Quan
Li, Xiao-ning
Li, Jun
Meng, Xian-Ming
Zhang, Hong-Yun
Liang, Jing-Jing
Li, Hui
Sun, Shi-Kai
Cai, Xin-Bin
Su, Li-Juan
Yin, Shan
Li, Yan-Sheng
Luo, Ting Rong
author_facet Feng, Li
Li, Xiao-Quan
Li, Xiao-ning
Li, Jun
Meng, Xian-Ming
Zhang, Hong-Yun
Liang, Jing-Jing
Li, Hui
Sun, Shi-Kai
Cai, Xin-Bin
Su, Li-Juan
Yin, Shan
Li, Yan-Sheng
Luo, Ting Rong
author_sort Feng, Li
collection PubMed
description BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear. RESULTS: In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001). CONCLUSION: We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF.
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spelling pubmed-34634352012-10-04 In vitro infection with classical swine fever virus inhibits the transcription of immune response genes Feng, Li Li, Xiao-Quan Li, Xiao-ning Li, Jun Meng, Xian-Ming Zhang, Hong-Yun Liang, Jing-Jing Li, Hui Sun, Shi-Kai Cai, Xin-Bin Su, Li-Juan Yin, Shan Li, Yan-Sheng Luo, Ting Rong Virol J Research BACKGROUND: Classical swine fever virus (CSFV) can evade the immune response and establish chronic infection under natural and experimental conditions. Some genes related to antigen processing and presentation and to cytokine regulation are known to be involved in this response, but the precise mechanism through which each gene responds to CSFV infection remains unclear. RESULTS: In this study, the amplification standard curve and corresponding linear regression equations for the genes SLA-2, TAP1, SLA-DR, Ii, CD40, CD80, CD86, IFN-α, and IFN-β were established successfully. Real-time RT-PCR was used to quantify the immune response gene transcription in PK-15 cells post CSFV infection. Results showed that: (1) immune response genes were generally down-regulated as a result of CSFV infection, and (2) the expression of SLA-2, SLA-DR, Ii and CD80 was significantly decreased (p<0.001). CONCLUSION: We conclude that in vitro infection with CSFV inhibits the transcription of host immune response genes. These findings may facilitate the development of effective strategies for controlling CSF. BioMed Central 2012-08-28 /pmc/articles/PMC3463435/ /pubmed/22925563 http://dx.doi.org/10.1186/1743-422X-9-175 Text en Copyright ©2012 Feng et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Feng, Li
Li, Xiao-Quan
Li, Xiao-ning
Li, Jun
Meng, Xian-Ming
Zhang, Hong-Yun
Liang, Jing-Jing
Li, Hui
Sun, Shi-Kai
Cai, Xin-Bin
Su, Li-Juan
Yin, Shan
Li, Yan-Sheng
Luo, Ting Rong
In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title_full In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title_fullStr In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title_full_unstemmed In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title_short In vitro infection with classical swine fever virus inhibits the transcription of immune response genes
title_sort in vitro infection with classical swine fever virus inhibits the transcription of immune response genes
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463435/
https://www.ncbi.nlm.nih.gov/pubmed/22925563
http://dx.doi.org/10.1186/1743-422X-9-175
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