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Distribution of MGEs and their insertion sites in the Macaca mulatta genome

Mobile genetic elements (MGEs) are fragments of DNA that can move around within the genome through retrotransposition. These are responsible for various important events such as gene inactivation, transduction, regulation of gene expression and genome expansion. The present work involves the identif...

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Autores principales: Rawal, Kamal, Priya, Avantika, Malik, Aman, Bahl, Radhika, Ramaswamy, Ram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463469/
https://www.ncbi.nlm.nih.gov/pubmed/23061019
http://dx.doi.org/10.4161/mge.21074
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author Rawal, Kamal
Priya, Avantika
Malik, Aman
Bahl, Radhika
Ramaswamy, Ram
author_facet Rawal, Kamal
Priya, Avantika
Malik, Aman
Bahl, Radhika
Ramaswamy, Ram
author_sort Rawal, Kamal
collection PubMed
description Mobile genetic elements (MGEs) are fragments of DNA that can move around within the genome through retrotransposition. These are responsible for various important events such as gene inactivation, transduction, regulation of gene expression and genome expansion. The present work involves the identification and study of the distribution of Alu and L1 retrotransposons in the genome of Macaca mulatta, an extensively used organism in biomedical studies. We also make comparisons with MGE distributions in other primate genomes and study the physicochemical properties of the local DNA structure around the transposon insertion site using ELAN. The present work also includes computational testing of the pre-insertion loci in order to detect unique features based on DNA structure, thermodynamic considerations and protein interaction measures. Although there is significant sequence divergence between the elements of M. mulatta and H. sapiens, their genome wide distribution is very similar; comparing the distribution of L1’s in all available X chromosome sequences suggests a common mechanism behind the spread of MGE’s in primate genomes.
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spelling pubmed-34634692012-10-11 Distribution of MGEs and their insertion sites in the Macaca mulatta genome Rawal, Kamal Priya, Avantika Malik, Aman Bahl, Radhika Ramaswamy, Ram Mob Genet Elements Research Paper Mobile genetic elements (MGEs) are fragments of DNA that can move around within the genome through retrotransposition. These are responsible for various important events such as gene inactivation, transduction, regulation of gene expression and genome expansion. The present work involves the identification and study of the distribution of Alu and L1 retrotransposons in the genome of Macaca mulatta, an extensively used organism in biomedical studies. We also make comparisons with MGE distributions in other primate genomes and study the physicochemical properties of the local DNA structure around the transposon insertion site using ELAN. The present work also includes computational testing of the pre-insertion loci in order to detect unique features based on DNA structure, thermodynamic considerations and protein interaction measures. Although there is significant sequence divergence between the elements of M. mulatta and H. sapiens, their genome wide distribution is very similar; comparing the distribution of L1’s in all available X chromosome sequences suggests a common mechanism behind the spread of MGE’s in primate genomes. Landes Bioscience 2012-05-01 /pmc/articles/PMC3463469/ /pubmed/23061019 http://dx.doi.org/10.4161/mge.21074 Text en Copyright © 2012 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Research Paper
Rawal, Kamal
Priya, Avantika
Malik, Aman
Bahl, Radhika
Ramaswamy, Ram
Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title_full Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title_fullStr Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title_full_unstemmed Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title_short Distribution of MGEs and their insertion sites in the Macaca mulatta genome
title_sort distribution of mges and their insertion sites in the macaca mulatta genome
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463469/
https://www.ncbi.nlm.nih.gov/pubmed/23061019
http://dx.doi.org/10.4161/mge.21074
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