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The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila

In animal gonads, PIWI proteins and their bound 23–30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches of piRNA biogenesis, namely primary processing and ping-pong amplification, have been proposed. Despite an overall conceptual understanding of piRNA...

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Autores principales: Olivieri, Daniel, Senti, Kirsten-André, Subramanian, Sailakshmi, Sachidanandam, Ravi, Brennecke, Julius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cell Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463805/
https://www.ncbi.nlm.nih.gov/pubmed/22902557
http://dx.doi.org/10.1016/j.molcel.2012.07.021
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author Olivieri, Daniel
Senti, Kirsten-André
Subramanian, Sailakshmi
Sachidanandam, Ravi
Brennecke, Julius
author_facet Olivieri, Daniel
Senti, Kirsten-André
Subramanian, Sailakshmi
Sachidanandam, Ravi
Brennecke, Julius
author_sort Olivieri, Daniel
collection PubMed
description In animal gonads, PIWI proteins and their bound 23–30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches of piRNA biogenesis, namely primary processing and ping-pong amplification, have been proposed. Despite an overall conceptual understanding of piRNA biogenesis, identity and/or function of the involved players are largely unknown. Here, we demonstrate an essential role for the female sterility gene shutdown in piRNA biology. Shutdown, an evolutionarily conserved cochaperone collaborates with Hsp90 during piRNA biogenesis, potentially at the loading step of RNAs into PIWI proteins. We demonstrate that Shutdown is essential for both primary and secondary piRNA populations in Drosophila. An extension of our study to previously described piRNA pathway members revealed three distinct groups of biogenesis factors. Together with data on how PIWI proteins are wired into primary and secondary processing, we propose a unified model for piRNA biogenesis.
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spelling pubmed-34638052012-11-01 The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila Olivieri, Daniel Senti, Kirsten-André Subramanian, Sailakshmi Sachidanandam, Ravi Brennecke, Julius Mol Cell Article In animal gonads, PIWI proteins and their bound 23–30 nt piRNAs guard genome integrity by the sequence specific silencing of transposons. Two branches of piRNA biogenesis, namely primary processing and ping-pong amplification, have been proposed. Despite an overall conceptual understanding of piRNA biogenesis, identity and/or function of the involved players are largely unknown. Here, we demonstrate an essential role for the female sterility gene shutdown in piRNA biology. Shutdown, an evolutionarily conserved cochaperone collaborates with Hsp90 during piRNA biogenesis, potentially at the loading step of RNAs into PIWI proteins. We demonstrate that Shutdown is essential for both primary and secondary piRNA populations in Drosophila. An extension of our study to previously described piRNA pathway members revealed three distinct groups of biogenesis factors. Together with data on how PIWI proteins are wired into primary and secondary processing, we propose a unified model for piRNA biogenesis. Cell Press 2012-09-28 /pmc/articles/PMC3463805/ /pubmed/22902557 http://dx.doi.org/10.1016/j.molcel.2012.07.021 Text en © 2012 ELL & Excerpta Medica. https://creativecommons.org/licenses/by-nc-nd/3.0/ Open Access under CC BY-NC-ND 3.0 (https://creativecommons.org/licenses/by-nc-nd/3.0/) license
spellingShingle Article
Olivieri, Daniel
Senti, Kirsten-André
Subramanian, Sailakshmi
Sachidanandam, Ravi
Brennecke, Julius
The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title_full The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title_fullStr The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title_full_unstemmed The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title_short The Cochaperone Shutdown Defines a Group of Biogenesis Factors Essential for All piRNA Populations in Drosophila
title_sort cochaperone shutdown defines a group of biogenesis factors essential for all pirna populations in drosophila
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463805/
https://www.ncbi.nlm.nih.gov/pubmed/22902557
http://dx.doi.org/10.1016/j.molcel.2012.07.021
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