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Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305

Introduction. Cirrhosis is a chronic degenerative illness characterized by changes in normal liver architecture, failure of hepatic function, and impairment of proliferative activity. The aim of this study is to know how IFC-305 compound induces proliferation of the liver during reversion of cirrhos...

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Autores principales: Chagoya de Sánchez, Victoria, Martínez-Pérez, Lidia, Hernández-Muñoz, Rolando, Velasco-Loyden, Gabriela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463961/
https://www.ncbi.nlm.nih.gov/pubmed/23056951
http://dx.doi.org/10.1155/2012/212530
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author Chagoya de Sánchez, Victoria
Martínez-Pérez, Lidia
Hernández-Muñoz, Rolando
Velasco-Loyden, Gabriela
author_facet Chagoya de Sánchez, Victoria
Martínez-Pérez, Lidia
Hernández-Muñoz, Rolando
Velasco-Loyden, Gabriela
author_sort Chagoya de Sánchez, Victoria
collection PubMed
description Introduction. Cirrhosis is a chronic degenerative illness characterized by changes in normal liver architecture, failure of hepatic function, and impairment of proliferative activity. The aim of this study is to know how IFC-305 compound induces proliferation of the liver during reversion of cirrhosis. Methods. Once cirrhosis has been installed by CCl(4) treatment for 10 weeks in male Wistar rats, they were divided into four groups: two received saline and two received the compound; all were euthanized at 5 and 10 weeks of treatment. Liver homogenate, mitochondria, and nucleus were used to measure cyclins, CDKs, and cell cycle regulatory proteins PCNA, pRb, p53, E2F, p21, p27, HGF, liver ATP, and mitochondrial function. Results. Diminution and small changes were observed in the studied proteins in the cirrhotic animals without treatment. The IFC-305-treated rats showed a clear increase in most of the proteins studied mainly in PCNA and CDK6, and a marked increased in ATP and mitochondrial function. Discussion/Conclusion. IFC-305 induces a recovery of the cell cycle inhibition promoting recovery of DNA damage through the action of PCNA and p53. The increase in energy and preservation of mitochondrial function contribute to recovering the proliferative function.
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spelling pubmed-34639612012-10-10 Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305 Chagoya de Sánchez, Victoria Martínez-Pérez, Lidia Hernández-Muñoz, Rolando Velasco-Loyden, Gabriela Int J Hepatol Research Article Introduction. Cirrhosis is a chronic degenerative illness characterized by changes in normal liver architecture, failure of hepatic function, and impairment of proliferative activity. The aim of this study is to know how IFC-305 compound induces proliferation of the liver during reversion of cirrhosis. Methods. Once cirrhosis has been installed by CCl(4) treatment for 10 weeks in male Wistar rats, they were divided into four groups: two received saline and two received the compound; all were euthanized at 5 and 10 weeks of treatment. Liver homogenate, mitochondria, and nucleus were used to measure cyclins, CDKs, and cell cycle regulatory proteins PCNA, pRb, p53, E2F, p21, p27, HGF, liver ATP, and mitochondrial function. Results. Diminution and small changes were observed in the studied proteins in the cirrhotic animals without treatment. The IFC-305-treated rats showed a clear increase in most of the proteins studied mainly in PCNA and CDK6, and a marked increased in ATP and mitochondrial function. Discussion/Conclusion. IFC-305 induces a recovery of the cell cycle inhibition promoting recovery of DNA damage through the action of PCNA and p53. The increase in energy and preservation of mitochondrial function contribute to recovering the proliferative function. Hindawi Publishing Corporation 2012 2012-09-27 /pmc/articles/PMC3463961/ /pubmed/23056951 http://dx.doi.org/10.1155/2012/212530 Text en Copyright © 2012 Victoria Chagoya de Sánchez et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chagoya de Sánchez, Victoria
Martínez-Pérez, Lidia
Hernández-Muñoz, Rolando
Velasco-Loyden, Gabriela
Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title_full Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title_fullStr Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title_full_unstemmed Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title_short Recovery of the Cell Cycle Inhibition in CCl(4)-Induced Cirrhosis by the Adenosine Derivative IFC-305
title_sort recovery of the cell cycle inhibition in ccl(4)-induced cirrhosis by the adenosine derivative ifc-305
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3463961/
https://www.ncbi.nlm.nih.gov/pubmed/23056951
http://dx.doi.org/10.1155/2012/212530
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