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Laser-induced inactivation of Plasmodium falciparum

BACKGROUND: Haemozoin crystals, produced by Plasmodium during its intra-erythrocytic asexual reproduction cycle, can generate UV light via the laser-induced, non-linear optical process of third harmonic generation (THG). In the current study the feasibility of using haemozoin, constitutively stored...

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Autores principales: LeBlanc, Danielle, Story, Robert, Gross, Eitan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464159/
https://www.ncbi.nlm.nih.gov/pubmed/22873646
http://dx.doi.org/10.1186/1475-2875-11-267
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author LeBlanc, Danielle
Story, Robert
Gross, Eitan
author_facet LeBlanc, Danielle
Story, Robert
Gross, Eitan
author_sort LeBlanc, Danielle
collection PubMed
description BACKGROUND: Haemozoin crystals, produced by Plasmodium during its intra-erythrocytic asexual reproduction cycle, can generate UV light via the laser-induced, non-linear optical process of third harmonic generation (THG). In the current study the feasibility of using haemozoin, constitutively stored in the parasite’s food vacuole, to kill the parasite by irradiation with a near IR laser was evaluated. METHODS: Cultured Plasmodium parasites at different stages of development were irradiated with a pulsed NIR laser and the viability of parasites at each stage was evaluated from their corresponding growth curves using the continuous culture method. Additional testing for germicidal effects of haemozoin and NIR laser was performed by adding synthetic haemozoin crystals to Escherichia coli in suspension. Cell suspensions were then irradiated with the laser and small aliquots taken and spread on agar plates containing selective agents to determine cell viability (CFU). RESULTS: Parasites in the late-trophozoites form as well as trophozoites in early-stage of DNA synthesis were found to be the most sensitive to the treatment with ~4-log reduction in viability after six passes through the laser beam; followed by parasites in ring phase (~2-log reduction). A ~1-log reduction in E. coli viability was obtained following a 60 min irradiation regimen of the bacteria in the presence of 1 μM synthetic haemozoin and a ~2-log reduction in the presence of 10 μM haemozoin. Minimal (≤15%) cell kill was observed in the presence of 10 μM haemin. CONCLUSIONS: Laser-induced third-harmonic generation by haemozoin can be used to inactivate Plasmodium. This result may have clinical implications for treating severe malaria symptoms by irradiating the patient’s blood through the skin or through dialysis tubing with a NIR laser.
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spelling pubmed-34641592012-10-05 Laser-induced inactivation of Plasmodium falciparum LeBlanc, Danielle Story, Robert Gross, Eitan Malar J Methodology BACKGROUND: Haemozoin crystals, produced by Plasmodium during its intra-erythrocytic asexual reproduction cycle, can generate UV light via the laser-induced, non-linear optical process of third harmonic generation (THG). In the current study the feasibility of using haemozoin, constitutively stored in the parasite’s food vacuole, to kill the parasite by irradiation with a near IR laser was evaluated. METHODS: Cultured Plasmodium parasites at different stages of development were irradiated with a pulsed NIR laser and the viability of parasites at each stage was evaluated from their corresponding growth curves using the continuous culture method. Additional testing for germicidal effects of haemozoin and NIR laser was performed by adding synthetic haemozoin crystals to Escherichia coli in suspension. Cell suspensions were then irradiated with the laser and small aliquots taken and spread on agar plates containing selective agents to determine cell viability (CFU). RESULTS: Parasites in the late-trophozoites form as well as trophozoites in early-stage of DNA synthesis were found to be the most sensitive to the treatment with ~4-log reduction in viability after six passes through the laser beam; followed by parasites in ring phase (~2-log reduction). A ~1-log reduction in E. coli viability was obtained following a 60 min irradiation regimen of the bacteria in the presence of 1 μM synthetic haemozoin and a ~2-log reduction in the presence of 10 μM haemozoin. Minimal (≤15%) cell kill was observed in the presence of 10 μM haemin. CONCLUSIONS: Laser-induced third-harmonic generation by haemozoin can be used to inactivate Plasmodium. This result may have clinical implications for treating severe malaria symptoms by irradiating the patient’s blood through the skin or through dialysis tubing with a NIR laser. BioMed Central 2012-08-08 /pmc/articles/PMC3464159/ /pubmed/22873646 http://dx.doi.org/10.1186/1475-2875-11-267 Text en Copyright ©2012 LeBlanc et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methodology
LeBlanc, Danielle
Story, Robert
Gross, Eitan
Laser-induced inactivation of Plasmodium falciparum
title Laser-induced inactivation of Plasmodium falciparum
title_full Laser-induced inactivation of Plasmodium falciparum
title_fullStr Laser-induced inactivation of Plasmodium falciparum
title_full_unstemmed Laser-induced inactivation of Plasmodium falciparum
title_short Laser-induced inactivation of Plasmodium falciparum
title_sort laser-induced inactivation of plasmodium falciparum
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464159/
https://www.ncbi.nlm.nih.gov/pubmed/22873646
http://dx.doi.org/10.1186/1475-2875-11-267
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