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In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride

Buccal films of ondanstron hydrochloride were fabricated from mucoadhesive polymer, chitosan, and polyvinyl pyrrolidone (PVP K30) for the purpose of prolonging drug release and improving its bioavailability. All fabricated film formulations prepared were smooth and translucent, with good flexibility...

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Autores principales: Koland, Marina, Charyulu, R Narayana, Vijayanarayana, K, Prabhu, Prabhakara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465139/
https://www.ncbi.nlm.nih.gov/pubmed/23071939
http://dx.doi.org/10.4103/2230-973X.85967
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author Koland, Marina
Charyulu, R Narayana
Vijayanarayana, K
Prabhu, Prabhakara
author_facet Koland, Marina
Charyulu, R Narayana
Vijayanarayana, K
Prabhu, Prabhakara
author_sort Koland, Marina
collection PubMed
description Buccal films of ondanstron hydrochloride were fabricated from mucoadhesive polymer, chitosan, and polyvinyl pyrrolidone (PVP K30) for the purpose of prolonging drug release and improving its bioavailability. All fabricated film formulations prepared were smooth and translucent, with good flexibility. The weight and thickness of all the formulations were found to be uniform. Drug content in the films ranged from 98 – 99%, indicating favorable drug loading and uniformity. The inclusion of PVP K30, a hydrophilic polymer, significantly reduced the bioadhesive strength and in vitro mucoadhesion time of the films, although the degree of swelling increased. In vitro drug release studies in simulated saliva showed a prolonged release of over five to six hours for all formulations, except C4, with 99.98% release in 1.5 hours. Kinetic analysis of the release data indicated that the best fit model with the highest correlation coefficient for all formulations was the Peppas model. In vivo studies, on selected films in rabbits, were conducted, to determine the pharmacokinetic parameters such as C(max), T(max), and AUC(0-∞), using model-independent methods with nonlinear least-squares regression analysis. The AUC and values of C(max) of ondansetron hydrochloride were found to be significantly greater (P < 0.005) than the selected films C2 and C3, as compared to those from the oral solution, thereby confirming improved bioavailability via the buccal route. The T(max) values were also significantly greater (P < 0.005), indicating the slower release of the drug from buccal films, thereby, providing prolonged effects. Good in vitro-in vivo correlation was observed with R(2) values exceeding 0.98, when the percentage of drug released was correlated with the percentage of drug absorbed.
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spelling pubmed-34651392012-10-15 In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride Koland, Marina Charyulu, R Narayana Vijayanarayana, K Prabhu, Prabhakara Int J Pharm Investig Original Research Article Buccal films of ondanstron hydrochloride were fabricated from mucoadhesive polymer, chitosan, and polyvinyl pyrrolidone (PVP K30) for the purpose of prolonging drug release and improving its bioavailability. All fabricated film formulations prepared were smooth and translucent, with good flexibility. The weight and thickness of all the formulations were found to be uniform. Drug content in the films ranged from 98 – 99%, indicating favorable drug loading and uniformity. The inclusion of PVP K30, a hydrophilic polymer, significantly reduced the bioadhesive strength and in vitro mucoadhesion time of the films, although the degree of swelling increased. In vitro drug release studies in simulated saliva showed a prolonged release of over five to six hours for all formulations, except C4, with 99.98% release in 1.5 hours. Kinetic analysis of the release data indicated that the best fit model with the highest correlation coefficient for all formulations was the Peppas model. In vivo studies, on selected films in rabbits, were conducted, to determine the pharmacokinetic parameters such as C(max), T(max), and AUC(0-∞), using model-independent methods with nonlinear least-squares regression analysis. The AUC and values of C(max) of ondansetron hydrochloride were found to be significantly greater (P < 0.005) than the selected films C2 and C3, as compared to those from the oral solution, thereby confirming improved bioavailability via the buccal route. The T(max) values were also significantly greater (P < 0.005), indicating the slower release of the drug from buccal films, thereby, providing prolonged effects. Good in vitro-in vivo correlation was observed with R(2) values exceeding 0.98, when the percentage of drug released was correlated with the percentage of drug absorbed. Medknow Publications & Media Pvt Ltd 2011 /pmc/articles/PMC3465139/ /pubmed/23071939 http://dx.doi.org/10.4103/2230-973X.85967 Text en Copyright: © International Journal of Pharmaceutical Investigation http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Koland, Marina
Charyulu, R Narayana
Vijayanarayana, K
Prabhu, Prabhakara
In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title_full In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title_fullStr In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title_full_unstemmed In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title_short In vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
title_sort in vitro and in vivo evaluation of chitosan buccal films of ondansetron hydrochloride
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465139/
https://www.ncbi.nlm.nih.gov/pubmed/23071939
http://dx.doi.org/10.4103/2230-973X.85967
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