A Facile and Specific Assay for Quantifying MicroRNA by an Optimized RT-qPCR Approach

BACKGROUND: The spatiotemporal expression patterns of microRNAs (miRNAs) are important to the verification of their predicted function. RT-qPCR is the accepted technique for the quantification of miRNA expression; however, stem-loop RT-PCR and poly(T)-adapter assay, the two most frequently used meth...

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Detalles Bibliográficos
Autores principales: Mei, Qian, Li, Xiang, Meng, Yuanguang, Wu, Zhiqiang, Guo, Mingzhou, Zhao, Yali, Fu, Xiaobing, Han, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465266/
https://www.ncbi.nlm.nih.gov/pubmed/23071657
http://dx.doi.org/10.1371/journal.pone.0046890

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