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Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice

Pneumoviruses such as pneumonia virus of mice (PVM), bovine respiratory syncytial virus (bRSV) or human (h)RSV are closely related pneumoviruses that cause severe respiratory disease in their respective hosts. It is well-known that T-cell responses are essential in pneumovirus clearance, but pneumov...

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Autores principales: van Helden, Mary J.G., van Kooten, Peter J.S., Bekker, Cornelis P.J., Gröne, Andrea, Topham, David J., Easton, Andrew J., Boog, Claire J.P., Busch, Dirk H., Zaiss, Dietmar M.W., Sijts, Alice J.A.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465553/
https://www.ncbi.nlm.nih.gov/pubmed/22940382
http://dx.doi.org/10.1016/j.vaccine.2012.08.027
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author van Helden, Mary J.G.
van Kooten, Peter J.S.
Bekker, Cornelis P.J.
Gröne, Andrea
Topham, David J.
Easton, Andrew J.
Boog, Claire J.P.
Busch, Dirk H.
Zaiss, Dietmar M.W.
Sijts, Alice J.A.M.
author_facet van Helden, Mary J.G.
van Kooten, Peter J.S.
Bekker, Cornelis P.J.
Gröne, Andrea
Topham, David J.
Easton, Andrew J.
Boog, Claire J.P.
Busch, Dirk H.
Zaiss, Dietmar M.W.
Sijts, Alice J.A.M.
author_sort van Helden, Mary J.G.
collection PubMed
description Pneumoviruses such as pneumonia virus of mice (PVM), bovine respiratory syncytial virus (bRSV) or human (h)RSV are closely related pneumoviruses that cause severe respiratory disease in their respective hosts. It is well-known that T-cell responses are essential in pneumovirus clearance, but pneumovirus-specific T-cell responses also are important mediators of severe immunopathology. In this study we determined whether memory- or pre-existing, transferred virus-specific CD8(+) T-cells provide protection against PVM-induced disease. We show that during infection with a sublethal dose of PVM, both natural killer (NK) cells and CD8(+) T-cells expand relatively late. Induction of CD8(+) T-cell memory against a single CD8(+) T-cell epitope, by dendritic cell (DC)-peptide immunization, leads to partial protection against PVM challenge and prevents Th2 differentiation of PVM-induced CD4 T-cells. In addition, adoptively transferred PVM-specific CD8(+) T-cells, covering the entire PVM-specific CD8(+) T-cell repertoire, provide partial protection from PVM-induced disease. From these data we infer that antigen-specific memory CD8(+) T-cells offer significant protection to PVM-induced disease. Thus, CD8(+) T-cells, despite being a major cause of PVM-associated pathology during primary infection, may offer promising targets of a protective pneumovirus vaccine.
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spelling pubmed-34655532012-11-06 Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice van Helden, Mary J.G. van Kooten, Peter J.S. Bekker, Cornelis P.J. Gröne, Andrea Topham, David J. Easton, Andrew J. Boog, Claire J.P. Busch, Dirk H. Zaiss, Dietmar M.W. Sijts, Alice J.A.M. Vaccine Article Pneumoviruses such as pneumonia virus of mice (PVM), bovine respiratory syncytial virus (bRSV) or human (h)RSV are closely related pneumoviruses that cause severe respiratory disease in their respective hosts. It is well-known that T-cell responses are essential in pneumovirus clearance, but pneumovirus-specific T-cell responses also are important mediators of severe immunopathology. In this study we determined whether memory- or pre-existing, transferred virus-specific CD8(+) T-cells provide protection against PVM-induced disease. We show that during infection with a sublethal dose of PVM, both natural killer (NK) cells and CD8(+) T-cells expand relatively late. Induction of CD8(+) T-cell memory against a single CD8(+) T-cell epitope, by dendritic cell (DC)-peptide immunization, leads to partial protection against PVM challenge and prevents Th2 differentiation of PVM-induced CD4 T-cells. In addition, adoptively transferred PVM-specific CD8(+) T-cells, covering the entire PVM-specific CD8(+) T-cell repertoire, provide partial protection from PVM-induced disease. From these data we infer that antigen-specific memory CD8(+) T-cells offer significant protection to PVM-induced disease. Thus, CD8(+) T-cells, despite being a major cause of PVM-associated pathology during primary infection, may offer promising targets of a protective pneumovirus vaccine. Elsevier Science 2012-10-05 /pmc/articles/PMC3465553/ /pubmed/22940382 http://dx.doi.org/10.1016/j.vaccine.2012.08.027 Text en © 2012 Elsevier Ltd. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
van Helden, Mary J.G.
van Kooten, Peter J.S.
Bekker, Cornelis P.J.
Gröne, Andrea
Topham, David J.
Easton, Andrew J.
Boog, Claire J.P.
Busch, Dirk H.
Zaiss, Dietmar M.W.
Sijts, Alice J.A.M.
Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title_full Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title_fullStr Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title_full_unstemmed Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title_short Pre-existing virus-specific CD8(+) T-cells provide protection against pneumovirus-induced disease in mice
title_sort pre-existing virus-specific cd8(+) t-cells provide protection against pneumovirus-induced disease in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465553/
https://www.ncbi.nlm.nih.gov/pubmed/22940382
http://dx.doi.org/10.1016/j.vaccine.2012.08.027
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