Cargando…

Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis

The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed t...

Descripción completa

Detalles Bibliográficos
Autores principales: Sakhno, Ludmila V., Tikhonova, Marina A., Tyrinova, Tamara V., Leplina, Olga Yu., Shevela, Ekaterina Ya., Nikonov, Sergey D., Zhdanov, Oleg A., Ostanin, Alexander A., Chernykh, Elena R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465906/
https://www.ncbi.nlm.nih.gov/pubmed/23056139
http://dx.doi.org/10.1155/2012/628635
_version_ 1782245609136193536
author Sakhno, Ludmila V.
Tikhonova, Marina A.
Tyrinova, Tamara V.
Leplina, Olga Yu.
Shevela, Ekaterina Ya.
Nikonov, Sergey D.
Zhdanov, Oleg A.
Ostanin, Alexander A.
Chernykh, Elena R.
author_facet Sakhno, Ludmila V.
Tikhonova, Marina A.
Tyrinova, Tamara V.
Leplina, Olga Yu.
Shevela, Ekaterina Ya.
Nikonov, Sergey D.
Zhdanov, Oleg A.
Ostanin, Alexander A.
Chernykh, Elena R.
author_sort Sakhno, Ludmila V.
collection PubMed
description The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed that dendritic cells (DCs), generated in vitro from patient blood monocytes with GM-CSF + IFN-α, were characterized by increased B7-H1 expression, upproduction of IL-10, and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). Moreover, DCs of patients with TB were able to enhance T cell apoptosis and to block T-cell division in MLC. It was shown that neutralizing anti-PD1 antibodies significantly decreased the proapoptogenic/tolerogenic effect of DCs. Correlation analysis revealed a direct relationship between IL-10 production and level of B7-H1 expression in the general group of investigated patients. It was demonstrated that generation of healthy donor DCs in the presence of IL-10 led to an increase in the number of DCs-expressed B7-H1 molecule, DC proapoptogenic activity, and a decrease in their allostimulatory activity. Obviously, the revealed phenomenon of the PD-1/B7-H1-mediated pro-apoptogenic activity of DCs is clinically significant since the cytotoxic/tolerogenic potential of DCs is more pronounced in patients with PPD anergy.
format Online
Article
Text
id pubmed-3465906
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-34659062012-10-10 Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis Sakhno, Ludmila V. Tikhonova, Marina A. Tyrinova, Tamara V. Leplina, Olga Yu. Shevela, Ekaterina Ya. Nikonov, Sergey D. Zhdanov, Oleg A. Ostanin, Alexander A. Chernykh, Elena R. Clin Dev Immunol Research Article The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed that dendritic cells (DCs), generated in vitro from patient blood monocytes with GM-CSF + IFN-α, were characterized by increased B7-H1 expression, upproduction of IL-10, and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). Moreover, DCs of patients with TB were able to enhance T cell apoptosis and to block T-cell division in MLC. It was shown that neutralizing anti-PD1 antibodies significantly decreased the proapoptogenic/tolerogenic effect of DCs. Correlation analysis revealed a direct relationship between IL-10 production and level of B7-H1 expression in the general group of investigated patients. It was demonstrated that generation of healthy donor DCs in the presence of IL-10 led to an increase in the number of DCs-expressed B7-H1 molecule, DC proapoptogenic activity, and a decrease in their allostimulatory activity. Obviously, the revealed phenomenon of the PD-1/B7-H1-mediated pro-apoptogenic activity of DCs is clinically significant since the cytotoxic/tolerogenic potential of DCs is more pronounced in patients with PPD anergy. Hindawi Publishing Corporation 2012 2012-09-29 /pmc/articles/PMC3465906/ /pubmed/23056139 http://dx.doi.org/10.1155/2012/628635 Text en Copyright © 2012 Ludmila V. Sakhno et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sakhno, Ludmila V.
Tikhonova, Marina A.
Tyrinova, Tamara V.
Leplina, Olga Yu.
Shevela, Ekaterina Ya.
Nikonov, Sergey D.
Zhdanov, Oleg A.
Ostanin, Alexander A.
Chernykh, Elena R.
Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title_full Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title_fullStr Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title_full_unstemmed Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title_short Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
title_sort cytotoxic activity of dendritic cells as a possible mechanism of negative regulation of t lymphocytes in pulmonary tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465906/
https://www.ncbi.nlm.nih.gov/pubmed/23056139
http://dx.doi.org/10.1155/2012/628635
work_keys_str_mv AT sakhnoludmilav cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT tikhonovamarinaa cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT tyrinovatamarav cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT leplinaolgayu cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT shevelaekaterinaya cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT nikonovsergeyd cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT zhdanovolega cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT ostaninalexandera cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis
AT chernykhelenar cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis