Cargando…
Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis
The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed t...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465906/ https://www.ncbi.nlm.nih.gov/pubmed/23056139 http://dx.doi.org/10.1155/2012/628635 |
_version_ | 1782245609136193536 |
---|---|
author | Sakhno, Ludmila V. Tikhonova, Marina A. Tyrinova, Tamara V. Leplina, Olga Yu. Shevela, Ekaterina Ya. Nikonov, Sergey D. Zhdanov, Oleg A. Ostanin, Alexander A. Chernykh, Elena R. |
author_facet | Sakhno, Ludmila V. Tikhonova, Marina A. Tyrinova, Tamara V. Leplina, Olga Yu. Shevela, Ekaterina Ya. Nikonov, Sergey D. Zhdanov, Oleg A. Ostanin, Alexander A. Chernykh, Elena R. |
author_sort | Sakhno, Ludmila V. |
collection | PubMed |
description | The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed that dendritic cells (DCs), generated in vitro from patient blood monocytes with GM-CSF + IFN-α, were characterized by increased B7-H1 expression, upproduction of IL-10, and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). Moreover, DCs of patients with TB were able to enhance T cell apoptosis and to block T-cell division in MLC. It was shown that neutralizing anti-PD1 antibodies significantly decreased the proapoptogenic/tolerogenic effect of DCs. Correlation analysis revealed a direct relationship between IL-10 production and level of B7-H1 expression in the general group of investigated patients. It was demonstrated that generation of healthy donor DCs in the presence of IL-10 led to an increase in the number of DCs-expressed B7-H1 molecule, DC proapoptogenic activity, and a decrease in their allostimulatory activity. Obviously, the revealed phenomenon of the PD-1/B7-H1-mediated pro-apoptogenic activity of DCs is clinically significant since the cytotoxic/tolerogenic potential of DCs is more pronounced in patients with PPD anergy. |
format | Online Article Text |
id | pubmed-3465906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-34659062012-10-10 Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis Sakhno, Ludmila V. Tikhonova, Marina A. Tyrinova, Tamara V. Leplina, Olga Yu. Shevela, Ekaterina Ya. Nikonov, Sergey D. Zhdanov, Oleg A. Ostanin, Alexander A. Chernykh, Elena R. Clin Dev Immunol Research Article The PD-1/B7-H1-mediated induction of T cell apoptosis/anergy as a possible mechanism of immune response failure was studied in 76 patients with pulmonary tuberculosis (TB) with normal and low-proliferative response to antigens of M. tuberculosis (purified protein derivative (PPD)). It was revealed that dendritic cells (DCs), generated in vitro from patient blood monocytes with GM-CSF + IFN-α, were characterized by increased B7-H1 expression, upproduction of IL-10, and reducing of allostimulatory activity in mixed lymphocyte culture (MLC). Moreover, DCs of patients with TB were able to enhance T cell apoptosis and to block T-cell division in MLC. It was shown that neutralizing anti-PD1 antibodies significantly decreased the proapoptogenic/tolerogenic effect of DCs. Correlation analysis revealed a direct relationship between IL-10 production and level of B7-H1 expression in the general group of investigated patients. It was demonstrated that generation of healthy donor DCs in the presence of IL-10 led to an increase in the number of DCs-expressed B7-H1 molecule, DC proapoptogenic activity, and a decrease in their allostimulatory activity. Obviously, the revealed phenomenon of the PD-1/B7-H1-mediated pro-apoptogenic activity of DCs is clinically significant since the cytotoxic/tolerogenic potential of DCs is more pronounced in patients with PPD anergy. Hindawi Publishing Corporation 2012 2012-09-29 /pmc/articles/PMC3465906/ /pubmed/23056139 http://dx.doi.org/10.1155/2012/628635 Text en Copyright © 2012 Ludmila V. Sakhno et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sakhno, Ludmila V. Tikhonova, Marina A. Tyrinova, Tamara V. Leplina, Olga Yu. Shevela, Ekaterina Ya. Nikonov, Sergey D. Zhdanov, Oleg A. Ostanin, Alexander A. Chernykh, Elena R. Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title | Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title_full | Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title_fullStr | Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title_full_unstemmed | Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title_short | Cytotoxic Activity of Dendritic Cells as a Possible Mechanism of Negative Regulation of T Lymphocytes in Pulmonary Tuberculosis |
title_sort | cytotoxic activity of dendritic cells as a possible mechanism of negative regulation of t lymphocytes in pulmonary tuberculosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3465906/ https://www.ncbi.nlm.nih.gov/pubmed/23056139 http://dx.doi.org/10.1155/2012/628635 |
work_keys_str_mv | AT sakhnoludmilav cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT tikhonovamarinaa cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT tyrinovatamarav cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT leplinaolgayu cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT shevelaekaterinaya cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT nikonovsergeyd cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT zhdanovolega cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT ostaninalexandera cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis AT chernykhelenar cytotoxicactivityofdendriticcellsasapossiblemechanismofnegativeregulationoftlymphocytesinpulmonarytuberculosis |